Methods and compositions for biomarkers of fatigue

ABSTRACT

The present invention provides methods and compositions for identifying fatigue, disease states associated with fatigue, recovery from fatigue and/or physical performance capability in a subject.

STATEMENT OF PRIORITY

This application is a continuation application of, and claims priority to, U.S. application Ser. No. 13/839,332, filed Mar. 15, 2013, which is a continuation-in-part application of, and claims priority to, PCT Application No. PCT/US2012/064798, filed Nov. 13, 2012, which claims the benefit, under 35 U.S.C. §119(e), of U.S. Provisional Application Ser. No. 61/559,632, filed Nov. 14, 2011, the entire contents of each of which are incorporated by reference herein.

FIELD OF THE INVENTION

The present invention relates to biomarkers and methods of their use in identifying fatigue, disease states associated with fatigue, recovery from fatigue and/or physical performance capability in a subject.

BACKGROUND OF THE INVENTION

There is great interest in finding methods that can be used to diagnosis, evaluate and/or monitor objectively the disease state referred to as chronic fatigue syndrome (CFS). As one example, an objective, saliva-based measurement tool would be useful in determining whether an individual is experiencing a level of fatigue sufficient to meet a diagnosis of chronic fatigue syndrome. Other applications include monitoring changes in fatigue level, e.g., in a subject diagnosed with CFS.

The present invention provides methods and compositions for diagnosing CFS, identifying subjects having an increased risk or likelihood of having or developing CFS and/or monitoring or evaluating fatigue in a subject by detecting and/or measuring biomarkers in one or more samples from the subject.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. For the biomarker bm_cfs_cand3, levels are greater in saliva from individuals with chronic fatigue syndrome (CFS) than in saliva from non-fatigued, control individuals (NF). The base 10 logarithm of the ion intensity, as determined by mass spectrometry, is shown as a function of patient type, i.e., CFS vs. NF. Data are shown as boxplots with the solid black line indicating the group median. The hollow box around the solid black line indicates the bounds of the data from the first to the third quartile. The whiskers indicate a distance 1.5× greater than the interquartile range from the nearest edge of the box. A non-parametric test suggested the two samples were unlikely to arise from a common distribution (Wilcoxon rank sum test, p<0.05).

FIG. 2. For the biomarker bm_cfs_cand4, levels are greater in saliva from individuals with chronic fatigue syndrome (CFS) than in saliva from non-fatigued, control individuals (NF). The base 10 logarithm of the ion intensity, as determined by mass spectrometry, is shown as a function of patient type, i.e., CFS vs. NF. Data are shown as boxplots with the solid black line indicating the group median. The hollow box around the solid black line indicates the bounds of the data from the first to the third quartile. The whiskers indicate a distance 1.5× greater than the interquartile range from the nearest edge of the box. A non-parametric test suggested the two samples were unlikely to arise from a common distribution (Wilcoxon rank sum test, p<0.05).

FIG. 3. For the biomarker sp_(—)6, levels are greater in saliva from individuals with chronic fatigue syndrome (CFS) than in saliva from non-fatigued, control individuals (NF). The base 10 logarithm of the ion intensity, as determined by mass spectrometry, is shown as a function of patient type, i.e., CFS vs. NF. Data are shown as boxplots with the solid black line indicating the group median. The hollow box around the solid black line indicates the bounds of the data from the first to the third quartile. The whiskers indicate a distance 1.5× greater than the interquartile range from the nearest edge of the box. A non-parametric test suggested the two samples were unlikely to arise from a common distribution (Wilcoxon rank sum test, p<0.05).

FIG. 4. A flow chart regarding the inclusion/exclusion of patients, showing procedure for obtaining 46 and 45 CFS and control saliva samples, respectively, from 21,165 subjects.

FIG. 5. CFS biomarker levels in CFS and normal subjects. Y-axis shows thousands of intensity units corresponding to concentration of CFS biomarker peptide in saliva normalized to total protein. Each circle represents a single sample.

FIG. 6. ROC curve for CFS salivary biomarker. Heavy solid line indicates sensitivity as a function of specificity (100-sensitivity). Dotted lines indicate 95% confidence interval. Light diagonal line indicates relationship between sensitivity and specificity if both CFS and control populations are the same. P-value associated with a comparison between the observed ROC AUC and ROC AUC=0.5 (no discrimination between diseased and normal) is <0.0001.

SUMMARY OF THE INVENTION

In some embodiments, the present invention provides a method of guiding a human subject's sleep schedule, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a sleep schedule; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's sleep schedule by modifying the duration of subsequent sleep periods using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent increase in the duration of the subject's sleep period, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change in the duration of the subject's sleep period or a subsequent decrease in the duration of the subject's sleep period.

In further embodiments, the present invention provides a method of guiding a human subject's use of a sleep enhancing material and/or sleep enhancing activity, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the sleep enhancing material and/or sleep enhancing activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's use of the sleep enhancing material and/or sleep enhancing activity using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent increase in the subject's use of the sleep enhancing material and/or sleep enhancing activity, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change or a subsequent decrease in the subject's use of the sleep enhancing material and/or sleep enhancing activity.

The present invention additionally provides a method of guiding a human subject's treatment of a sleep disorder, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) treating the subject for the sleep disorder; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's treatment of the sleep disorder using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent enhancement of the treatment for the sleep disorder, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change or a subsequent reduction of the treatment for the sleep disorder.

Further provided herein is a method of identifying a substance and/or activity that enhances sleep, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the test substance and/or test activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) determining if the test substance and/or test activity enhances sleep using the subject's ratio(s) as calculated in (e), such that a decrease in the ratio relative to the previous ratio identifies the test substance and/or test activity as a substance and/or an activity that enhances sleep.

In addition, the present invention provides a method of identifying a substance and/or activity that treats a sleep disorder, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the test substance and/or test activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) determining if the test substance and/or test activity treats the sleep disorder using the subject's ratio(s) as calculated in (e), such that a decrease in the ratio relative to the previous ratio identifies the test substance and/or test activity as a substance and/or an activity that treats the sleep disorder.

A method is also provided herein of identifying a human subject that is sleep deprived, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from each subject in a population of subjects that are not sleep deprived; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in each sample of (a), according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for each of the study subjects in the population of (a); c) establishing a threshold ratio for the population of subjects of (a); d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a test subject; e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the test subject; and f) comparing the ratio of the test subject with the threshold ratio of (c), whereby a ratio of the test subject that is greater than the threshold ratio of (c) identifies the test subject as being sleep deprived.

Also provided herein is a method of identifying a human subject that is sleep deprived, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the subject; and c) comparing the ratio of the subject with a threshold ratio, whereby a ratio of the subject that is greater than the threshold ratio identifies the subject as sleep deprived.

In additional embodiments, the present invention provides a method of guiding a human subject's treatment of fatigue, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) treating the subject for fatigue; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's treatment of fatigue using the subject's ratio(s) as calculated in (e), such that a an increase in the ratio relative to the previous ratio leads to a subsequent enhancement of the treatment for fatigue, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change or a subsequent reduction of the treatment for fatigue.

Further provided herein is a method of identifying a substance and/or activity that reduces fatigue, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the test substance and/or test activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) determining if the test substance and/or test activity reduces fatigue using the subject's ratio(s) as calculated in (e), such that a decrease in the ratio relative to the previous ratio identifies the test substance and/or test activity as a substance and/or an activity that reduces fatigue.

Additionally provided herein is a method of guiding a human subject's treatment of a chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) treating the subject for CFS; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's treatment of CFS using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent enhancement of the treatment for CFS, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change or a subsequent reduction of the treatment for CFS.

The present invention also provides a method of identifying a substance and/or activity that treats chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the test substance and/or test activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point after step (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) determining if the test substance and/or test activity treats CFS using the subject's ratio(s) as calculated in (e), such that a decrease in the ratio relative to the previous ratio identifies the test substance and/or test activity as a substance and/or an activity that treats CFS.

Additionally provided herein is a method of identifying a human subject having an increased likelihood of having or developing chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from each subject in a population of subjects that do not have a diagnosis of CFS or symptoms of CFS; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in each sample of (a), according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for each of the study subjects in the population of (a); c) establishing a threshold ratio for the population of subjects of (a); d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a test subject; e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the test subject; and f) comparing the ratio of the test subject with the threshold ratio of (c), whereby a ratio of the test subject that is greater than the threshold ratio of (c) identifies the subject as having an increased likelihood of having or developing CFS.

In further aspects of this invention, a method is provided of identifying a human subject having an increased likelihood of having or developing chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the subject; and c) comparing the ratio of the subject with a threshold ratio, whereby a ratio of the subject that is greater than the threshold ratio identifies the subject as having an increased likelihood of having or developing CFS.

Additionally provided herein is a method of guiding a human subject's work load, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a work load; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's work load by modifying the duration of the subject's work period and/or amount of work the subject does using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent decrease in the duration of the subject's work period and/or a decrease in the amount of work the subject does, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change in the duration of the subject's work period and/or amount of work the subject does or a subsequent increase in the duration of the subject's work period and/or amount of work the subject does.

In further aspects, the present invention provides a method of identifying a human subject sufficiently rested to carry out a work load, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from each subject in a population of subjects that have carried out the work load sufficiently, wherein the sample is taken from each subject at about the time the subject starts the work load; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for each of subject in the population of subjects of (a); c) establishing a threshold ratio for the population of subjects of (a); d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a test subject; e) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the test subject; and f) comparing the ratio of the test subject with the threshold ratio of (c), whereby a ratio of the test subject that is less than or equal to the threshold ratio of (c) identifies the test subject as being sufficiently rested to carry out the work load and a ratio of the test subject that is greater than the threshold ratio of (c) identifies the test subject as not being sufficiently rested to carry out the workload.

Additionally provided herein is a method of identifying a human subject that is sufficiently rested to carry out a work load, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the subject; and c) comparing the ratio of the subject with a threshold ratio, whereby a ratio of the subject that is less than or equal to the threshold ratio identifies the subject as being sufficiently rested to carry out the work load and a ratio of the subject that is greater than the threshold ratio identifies the subject as not being sufficiently rested to carry out the work load.

Additionally, the present invention provides a method of identifying a human subject who is fit for duty, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from each subject in a population of subjects that are fit for duty: b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for each subject in the population of subjects of (a); c) establishing a threshold ratio for the population of subjects of (a); d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a test subject; e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the test subject; and f) comparing the ratio of the test subject with the threshold ratio of (c), whereby a ratio of the test subject that is greater than the threshold ratio of (c) identifies the test subject as being not fit for duty, and a ratio of the test subject that is the same as or less than the threshold ratio of (c) identifies the test subject as being fit for duty.

Furthermore, the present invention provides a method of identifying a human subject who is fit for duty, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg) to determine a ratio for the subject; and c) comparing the ratio of the subject with a threshold ratio, whereby a ratio of the subject that is greater than the threshold ratio identifies the subject as being not fit for duty, and a ratio of the subject that is the same as or less than the threshold ratio identifies the subject as being fit for duty.

In a further aspect, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (non-CFS subjects); b) calculating the amount of the peptide relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ in a biological sample from a test subject; and e) calculating the amount of the peptide relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In an additional aspect, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the peptide relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ in a biological sample from a test subject; and e) calculating the amount of the peptide relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

A further aspect of this invention is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the peptide relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject; and e) calculating the amount of the peptide relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Further provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of each of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the peptides relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject of the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of each of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject; and e) calculating the amount of the peptides relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Also provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome; b) calculating the amount of the peptides relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject of the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject (e.g., wherein the peptides of (d) are the same as peptides as measured in (a)); and e) calculating the amount of the peptides relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

An additional aspect of this invention includes a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 1 (PRB1) in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of PRB1 relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of human basic proline-rich protein 1 (PRB1) in a biological sample from a test subject; and e) calculating the amount of PRB1 relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Other aspects of this invention include a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 2 (PRB2) in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of PRB2 relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of human basic proline-rich protein 2 (PRB2) in a biological sample from a test subject; and e) calculating the amount of PRB2 relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

The present invention also provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 4 (PRB4) in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of PRB4 relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of human basic proline-rich protein 4 (PRB4) in a biological sample from a test subject; and e) calculating the amount of PRB4 relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Furthermore, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of each of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the proteins relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a biological sample from a test subject; and e) calculating the amount of the proteins relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Additionally provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more proteins selected from the group consisting of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome; b) calculating the amount of the proteins relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from a test subject, (e.g., wherein the proteins of (d) are the same proteins as measured in (a)); and e) calculating the amount of the proteins relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In further embodiments, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ in a biological sample from a test subject; and b) calculating the amount of the peptide relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In addition, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ in a biological sample from a test subject; and b) calculating the amount of the peptide relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Also provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject; and b) calculating the amount of the peptide relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Furthermore, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject; and b) calculating the amount of the peptides relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

The present invention also provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in a biological sample from a test subject; and b) calculating the amount of the peptides relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Additionally, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 1 (PRB1) in a biological sample from a test subject; and b) calculating the amount of PRB1 relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In yet further embodiments, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 2 (PRB2) in a biological sample from a test subject; and b) calculating the amount of PRB2 relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Further provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of human basic proline-rich protein 4 (PRB4) in a biological sample from a test subject; and b) calculating the amount of PRB4 relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

The present invention also provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a biological sample from a test subject; and b) calculating the amount of the proteins relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Furthermore, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from a test subject; and b) calculating the amount of the proteins relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Additional aspects of this invention include a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ in a first biological sample from the subject; b) calculating the amount of the peptide of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of the peptide comprising an amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, in a second or subsequent biological sample(s); and d) calculating the amount of the peptide of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

The present invention further provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, in a first biological sample from the subject; b) calculating the amount of the peptide of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of the peptide comprising an amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ in a second or subsequent biological sample(s); and d) calculating the amount of the peptide of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

Also provided herein is a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a first biological sample from the subject; b) calculating the amount of the peptide of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of the peptide comprising an amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a second or subsequent biological sample(s); and d) calculating the amount of the peptide of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

Furthermore, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a first biological sample from the subject; b) calculating the amount of the peptides of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a second or subsequent biological sample(s); and d) calculating the amount of the peptides of (c) relative to the total amount of the protein in the respective sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

Furthermore, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a first biological sample from the subject; b) calculating the amount of the peptides of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a second or subsequent biological sample(s) (e.g., wherein the peptides measured in (c) are the same peptides as measured in (a)); and d) calculating the amount of the peptides of (c) relative to the total amount of the protein in the respective sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In an additional aspect, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of human basic proline-rich protein 1 (PRB1) in a first biological sample from the subject; b) calculating the amount of the protein of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of human basic proline-rich protein 1 (PRB1) in a second or subsequent biological sample(s); and d) calculating the amount of the protein of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In a further aspect, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of human basic proline-rich protein 2 (PRB2) in a first biological sample from the subject; b) calculating the amount of the protein of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of human basic proline-rich protein 2 (PRB2) in a second or subsequent biological sample(s); and d) calculating the amount of the protein of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

Further provided herein is a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of human basic proline-rich protein 4 (PRB4) in a first biological sample from the subject; b) calculating the amount of the protein of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s); and d) calculating the amount of the protein of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In an additional aspect of this invention, a method is provided, of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a first biological sample from the subject; b) calculating the amount of the proteins of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s); and d) calculating the amount of the proteins of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In a further aspect of this invention, a method is provided, of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a first biological sample from the subject; b) calculating the amount of the proteins of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s) (e.g., wherein the peptides measured in (c) are the same peptides as measured in (a); and d) calculating the amount of the proteins of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In yet further embodiments, the present invention provides a method of guiding a human subject's treatment for chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a treatment program for chronic fatigue syndrome; d) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample of (d) according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's treatment for chronic fatigue syndrome by modifying the intensity of subsequent treatments using the latest of the subject's ratio(s) as calculated in (e), such that an increase in chronic fatigue relative to the previous measurement of chronic fatigue leads to a subsequent increase in the subject's treatment intensity.

Also provided herein is a method of evaluating the effect of a treatment material and/or activity on the chronic fatigue level of a human subject (e.g., a subject diagnosed as having, determined to have or suspected of having chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a subject at a time point prior to exposing the subject to a treatment material and/or activity; b) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the treatment material and/or performance enhancing activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) evaluating the effect of the treatment material and/or activity on the chronic fatigue status of the subject by comparing the ratios of (b) and (e), wherein an increase in the ratio of (e) relative to the ratio of (b) is indicative of increased chronic fatigue of the subject, a decrease in the ratio of (e) relative to the ratio of (b) is indicative of decreased chronic fatigue of the subject, and a constant ratio of (e) relative to the ratio of (b) is indicative of no change in chronic fatigue of the subject.

Additionally provided herein is a method of guiding a human subject's physical training activity, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject when the subject is in a rested state, wherein the subject is an adult athlete or an amateur athlete; b) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a physical training program comprising activities of different intensity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's physical training program by modifying the intensity of subsequent activities using the latest of the subject's ratio(s), as calculated in (e), such that a decrease in the ratio relative to the previous ratio leads to a subsequent increase in the subject's training intensity, an increase in the ratio relative to the previous ratio leads to a subsequent decrease in the subject's training intensity, and a constant ratio relative to the previous ratio leads to a subsequent constant level in the subject's training intensity. In some embodiments, the physical training program can be a military training program.

The present invention also provides a method of evaluating the effect of a performance enhancing material and/or activity on the physical performance capability of a human subject, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point prior to exposing the subject to a performance enhancing material or performance enhancing activity, wherein the subject is an adult athlete or amateur athlete; b) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the performance enhancing material and/or performance enhancing activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) evaluating the effect of the performance enhancing material and/or activity on the physical performance capability of the subject by comparing the ratios of (b) and (e), wherein an increase in the ratio of (e) relative to the ratio of (b) is indicative of reduced physical performance capability of the subject, a decrease in the ratio of (e) relative to the ratio of (b) is indicative of improved physical performance capability of the subject, and a constant ratio of (e) relative to the ratio of (b) is indicative of no change in physical performance capability of the subject.

DETAILED DESCRIPTION OF THE INVENTION

As used herein, “a” or “an” or “the” can mean one or more than one. For example, “a” cell can mean one cell or a plurality of cells.

Also as used herein, “and/or” refers to and encompasses any and/or all possible combinations of one or more of the associated listed items, as well as the lack of and and/or combinations when interpreted in the alternative (“or”).

Furthermore, the term “about” as used herein when referring to a measurable value such as an amount of a compound or agent of this invention, dose, time, temperature, and the like, is meant to encompass variations of ±20%, ±10%, ±5%, ±1%, ±0.5%, or even ±0.1% of the specified amount.

The present invention is explained in greater detail below. This description is not intended to be a detailed catalog of all the different ways in which the invention may be implemented, or all the features that may be added to the instant invention. For example, features illustrated with respect to one embodiment may be incorporated into other embodiments, and features illustrated with respect to a particular embodiment may be deleted from that embodiment. In addition, numerous variations and additions to the various embodiments suggested herein will be apparent to those skilled in the art in light of the instant disclosure, which do not depart from the instant invention. Hence, the following specification is intended to illustrate some particular embodiments of the invention, and not to exhaustively specify all permutations, combinations and variations thereof.

The present invention is based on the unexpected discovery of a modulation of biomarkers (e.g., modulation of peptide and/or protein levels) as measured in a biological sample from a subject that correlate with the subject's status with regard to having chronic fatigue syndrome, having an increased risk or likelihood of having or developing chronic fatigue syndrome and/or having an altered (e.g., increased or decreased) level of fatigue over time.

Thus, in one embodiment, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the peptide(s) relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject of the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a biological sample from a test subject; and e) calculating the amount of the peptide(s) relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In a further embodiment, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4), in any combination, in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects); b) calculating the amount of the protein(s) relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4), in any combination, in a biological sample from a test subject; and e) calculating the amount of the protein(s) relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In an additional embodiment, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a first biological sample from the subject; b) calculating the amount of the peptide(s) of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a second or subsequent biological sample(s); and d) calculating the amount of the peptide(s) of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

Furthermore, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a biological sample from a test subject; and b) calculating the amount of the peptide(s) relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

The present invention also provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in any combination, in a biological sample from a test subject; and b) calculating the amount of the protein(s) relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

In an additional embodiment of this invention, a method is provided, of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4), in any combination, in a first biological sample from the subject; b) calculating the amount of the protein(s) of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s); and d) calculating the amount of the protein(s) of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In addition, the present invention provides a method of identifying an increase over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a first biological sample from the subject; b) calculating the amount of the peptide(s) of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in any combination, in a second or subsequent biological sample(s); and d) calculating the amount of the peptide(s) of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein an increase in the second or subsequent biomarker index relative to the first biomarker index identifies an increase over time in the fatigue level of the subject.

In an additional embodiment of this invention, a method is provided, of identifying an increase over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4), in any combination, in a first biological sample from the subject; b) calculating the amount of the protein(s) of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s); and d) calculating the amount of the protein(s) of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein an increase in the second or subsequent biomarker index relative to the first biomarker index identifies an increase over time in the fatigue level of the subject.

Also provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome; b) calculating the amount of the peptides relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject of the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a biological sample from a test subject (e.g., wherein the peptides of (d) are the same as peptides as measured in (a)); and e) calculating the amount of the peptides relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Additionally provided herein is a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more proteins selected from the group consisting of 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from each subject in a population of subjects determined not to have chronic fatigue syndrome; b) calculating the amount of the proteins relative to the total amount of protein in each sample of (a) to determine a biomarker index for each subject in the population; c) establishing a threshold biomarker index from the biomarker indices determined in (b); d) measuring the amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from a test subject, (e.g., wherein the proteins of (d) are the same proteins as measured in (a)); and e) calculating the amount of the proteins relative to the total amount of protein in the sample of (d) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than the threshold biomarker index of (c) identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

The present invention also provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, in a biological sample from a test subject; and b) calculating the amount of the peptides relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Furthermore, the present invention provides a method of identifying a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome, comprising: a) measuring the amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4), in a biological sample from a test subject; and b) calculating the amount of the proteins relative to the total amount of protein in the sample of (a) to determine a biomarker index for the test subject, wherein a biomarker index of the test subject that is higher than a threshold biomarker index identifies the subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome.

Furthermore, the present invention provides a method of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of two or more peptides selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a first biological sample from the subject; b) calculating the amount of the peptides of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ in a second or subsequent biological sample(s) (e.g., wherein the peptides measured in (c) are the same peptides as measured in (a)); and d) calculating the amount of the peptides of (c) relative to the total amount of the protein in the respective sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In a further aspect of this invention, a method is provided, of identifying a decrease over time in fatigue level of a subject, comprising: a) measuring, at a first time point, an amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a first biological sample from the subject; b) calculating the amount of the proteins of (a) relative to the total amount of protein in the first sample to determine a first biomarker index for the subject at the first time point; c) measuring, at a second or subsequent time point(s), an amount of two or more proteins selected from the group consisting of: 1) human basic proline-rich protein 1 (PRB1), 2) human basic proline-rich protein 2 (PRB2), and 3) human basic proline-rich protein 4 (PRB4) in a second or subsequent biological sample(s) (e.g., wherein the peptides measured in (c) are the same peptides as measured in (a); and d) calculating the amount of the proteins of (c) relative to the total amount of the protein in the second or subsequent sample(s) to determine a second or subsequent biomarker index for the subject at the second or subsequent time point(s), wherein a decrease in the second or subsequent biomarker index relative to the first biomarker index identifies a decrease over time in the fatigue level of the subject.

In yet further embodiments, the present invention provides a method of guiding a human subject's treatment for chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a treatment program for chronic fatigue syndrome; d) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample of (d) according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's treatment for chronic fatigue syndrome by modifying the intensity of subsequent treatments using the latest of the subject's ratio(s) as calculated in (e), such that an increase in chronic fatigue relative to the previous measurement of chronic fatigue leads to a subsequent increase in the subject's treatment intensity.

Also provided herein is a method of evaluating the effect of a treatment material and/or activity on the chronic fatigue level of a human subject (e.g., a subject diagnosed as having, determined to have or suspected of having chronic fatigue syndrome (CFS), comprising: a) measuring the concentration of a peptide selected from the group consisting of 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from a subject at a time point prior to exposing the subject to a treatment material and/or activity; b) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the treatment material and/or performance enhancing activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of chronic fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) evaluating the effect of the treatment material and/or activity on the chronic fatigue status of the subject by comparing the ratios of (b) and (e), wherein an increase in the ratio of (e) relative to the ratio of (b) is indicative of increased chronic fatigue of the subject, a decrease in the ratio of (e) relative to the ratio of (b) is indicative of decreased chronic fatigue of the subject, and a constant ratio of (e) relative to the ratio of (b) is indicative of no change in chronic fatigue of the subject.

Additionally provided herein is a method of guiding a human subject's physical training activity, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject when the subject is in a rested state, wherein the subject is an adult athlete or an amateur athlete; b) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a physical training program comprising activities of different intensity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's physical training program by modifying the intensity of subsequent activities using the latest of the subject's ratio(s), as calculated in (e), such that a decrease in the ratio relative to the previous ratio leads to a subsequent increase in the subject's training intensity, an increase in the ratio relative to the previous ratio leads to a subsequent decrease in the subject's training intensity, and a constant ratio relative to the previous ratio leads to a subsequent constant level in the subject's training intensity. In some embodiments, the physical training program can be a military training program.

The present invention also provides a method of evaluating the effect of a performance enhancing material and/or activity on the physical performance capability of a human subject, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at a time point prior to exposing the subject to a performance enhancing material or performance enhancing activity, wherein the subject is an adult athlete or amateur athlete; b) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) exposing the subject to the performance enhancing material and/or performance enhancing activity; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), a 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) identifying the subject's level of physical fatigue by calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) evaluating the effect of the performance enhancing material and/or activity on the physical performance capability of the subject by comparing the ratios of (b) and (e), wherein an increase in the ratio of (e) relative to the ratio of (b) is indicative of reduced physical performance capability of the subject, a decrease in the ratio of (e) relative to the ratio of (b) is indicative of improved physical performance capability of the subject, and a constant ratio of (e) relative to the ratio of (b) is indicative of no change in physical performance capability of the subject.

In methods wherein the level of more than one peptide or protein is measured for a subject, the biomarker index for the subject could be determined by any combination of the peptide or protein data. For example, the biomarker index might be a simple sum of the different peptide or protein levels, e.g., simple addition of the peptide or protein levels and division of the sum by the total amount of protein in the sample. More complex combinations of peptide or protein data might also be used. For example, the biomarker index could be calculated as a weighted sum of the various peptides or proteins, whereby the measured peptide or protein levels are multiplied by independent weighting factors before the values are summed; it is possible that one of the weighting factors could be zero. Higher-order mathematical combinations of peptide or protein levels might also be considered. For example, an equation for calculating the biomarker index might include a squared, cubed, or other higher-order term for one or more of the various peptides or proteins.

As used herein, “biomarker” can mean any chemical or biological entity that is produced by cells and/or by commensal flora, or substances that are produced by cells or commensal flora that might be then chemically modified by extracellular enzymes, free radicals produced by cells of the body and/or other naturally occurring processes and that is found, for example, in the saliva, urine, blood, vaginal secretion, tears, feces, sputum, hair, nails, skin, wound fluid, nasal swab, lymph, perspiration, oral mucosa, vaginal mucosa, or the anus, or in serum or plasma obtained from blood. Thus, in the methods of this invention, the sample can be any biological fluid or tissue that can be used in an assay of this invention, including but not limited to, serum, plasma, blood, saliva, semen, lymph, cerebrospinal fluid, prostatic fluid, urine, sputum, oral mucosa, nasal mucosa, duodenal fluid, gastric fluid, skin, endothelium, biopsy material from a salivary gland, biopsy material of a parotid gland, biopsy material of other glands of the mouth, secretions of the salivary gland, secretions of the parotid gland, secretions of other glands of the mouth, joint fluid, body cavity fluid, tear fluid, anal secretions; vaginal secretions, perspiration, whole cells, cell extracts, tissue, biopsy material, aspirates, exudates, slide preparations, fixed cells, tissue sections, etc.

In various embodiments of this invention, the biological sample can be prepared according to methods well known in the art and as described in the Examples section herein, to be a small molecular weight (SMW) sample. In particular embodiments of this invention, the biological sample is saliva.

In some embodiments, a biomarker of this invention can be, but is not limited to, a peptide or polypeptide comprising, consisting essentially of and/or consisting of the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ, the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ, and/or the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ, singly or in any combination. In some embodiments, the biomarker of this invention can be, but is not limited to human basic proline-rich protein 1 (PRB1), human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4), the amino acid sequence and nucleotide sequence of each of which is known in the art, as exemplified in Table 2.

In further embodiments, a biomarker of this invention can be a peptide comprising, consisting essentially of or consisting of any fragment of human basic proline-rich protein 1 (PRB1), human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4). For example, the biomarker of this invention can be a peptide comprising, consisting essentially of or consisting of one or more 5 mers, 6 mers, 7 mers, 8 mers, 9 mers or 10 mers of the amino acid sequence of any of human basic proline-rich protein 1 (PRB1), human basic proline-rich protein 2 (PRB2), and/or 3) human basic proline-rich protein 4 (PRB4).

As a nonlimiting example, a biomarker of this invention can be a peptide comprising, consisting essentially of or consisting of at least about five amino acids (e.g., 5, 6, 7, 8, 9, 10, 11, 12, 13, 14) to at least about 15, 20, 25, 30, 35, 40, 45, 50, 50, 70, 80, 90, 100, 125, 150, 175 or 200 amino acids (e.g., 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 1120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, etc.), wherein the peptide comprises one or more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20) of the 5 mer peptides listed in Table 1. As a nonlimiting example, a biomarker peptide of this invention can be 20 amino acids in length and can comprise two of the 5 mer peptides of Table 1, which can be contiguous as provided in the protein sequence from which the 5 mer peptides were derived. The remaining 10 amino acids can be on either side of each 5 mer, between each 5 mer or both. Furthermore, the remaining amino acids can be amino acids that are contiguous with the 5 mer peptides and/or noncontiguous with the 5 mer peptides (e.g., amino acids that are not present in the order provided in the protein sequence from which the 5 mer peptides were derived). The 5 mers of the PRB proteins provided in Table 1 are exemplary, as the present invention also encompasses 6 mers, 7 mers, 8 mers, 9 mers, etc., of these PRB proteins as noted above, the amino acid sequence of any of which would be readily determined by one of skill in the art.

In further embodiments, a biomarker of this invention can comprise, consist essentially of or consist of any peptide or protein listed in Table 1, singly or in any combination. The biomarker of this invention can also comprise, consist essentially of or consist of any fragment of any peptide or protein listed in Table 1.

Thus, in certain embodiments, the present invention is directed to a biomarker, which is a peptide or polypeptide (i.e., protein) as described herein and the present invention can employ or involve one or more of the peptides and proteins set forth herein in any method and/or kit of this invention, singly and/or in any combination. In certain other embodiments, the present invention provides a nucleic acid encoding a biomarker of this invention and the methods of this invention can employ or involve one or more of the nucleic acids of this invention in any method/and or kit of this invention.

A biomarker of this invention can be detected and/or quantified in a sample by a variety of methods well known in the art for detecting and/or quantifying substances in biological samples. For example, for detecting and/or quantifying a biomarker that is a peptide or polypeptide, standard methods for detecting and/or quantifying peptides and/or polypeptides in sample can be employed. Nonlimiting examples of such methods include direct protein measurement, absorbance at 280 nm, absorbance at 205 nm, extinction coefficient assay, Lowry assay, biuret assay, Bradford assay, bicinchoninic acid assay (BCA), amido black assay, colloidal gold assay, immunoassay or other specific binding assay employing an antibody or ligand that specifically binds a peptide or polypeptide, protein separation assays such as electrophoresis, gas chromatography (GC), high performance liquid chromatography (HPLC), mass spectrometry (MS), etc., as are well known in the art.

Other methods of detection can include bioassays using mammalian or bacterial cells wherein an output is proportional to the concentration of peptide in the sample solution, and solid phase methods wherein binding to a surface coated with a peptide recognizing molecule triggers an output electrical signal or change in optical property.

In some embodiments of this invention, peptides and/or proteins in a sample of this invention can be measured by BCA.

For detection and/or quantification of a nucleic acid encoding a biomarker of this invention, standard methods for detection and/or quantification of nucleic acids in a sample can be employed. Non-limiting examples include hybridization assays, amplification assays, sequencing protocols, etc., as are well known in the art.

The term “biomarker index” as used herein means the ratio of the amount of biomarker peptide or protein to the total amount of protein in the biological sample. For example, the biomarker index can be a value determined by calculating the relative amount of peptide in nmoles per μg of total protein (e.g., as determined by BCA) in the biological sample. The term “threshold biomarker index” as used herein means the biomarker index calculated from the biomarker indices from a population of subjects that defines the threshold value for identifying a subject as having chronic fatigue syndrome or having an increased likelihood or risk of having or developing chronic fatigue syndrome. The threshold biomarker index is calculated from the biomarker index of each subject in a population of subjects determined not to have chronic fatigue syndrome (e.g., non-CFS subjects). In some embodiments, a predetermined or previously established threshold biomarker index can be used in the methods described herein to identify a subject as having chronic fatigue syndrome or having an increased likelihood of having or developing chronic fatigue syndrome. Such a predetermined or previously established threshold biomarker index can be determined according to the teachings set forth herein (see, e.g., Example 3) as well as art-known teachings.

A subject of this invention is any animal in which identification of chronic fatigue syndrome, identification of increased likelihood or risk of having or developing chronic fatigue syndrome and/or identification of changes in fatigue level over time is needed or desired. In some embodiments, the subject is mammal and in particular embodiments the subject is a human. In other embodiments, the subject can be a horse, a dog or any other mammal about which the information obtained from the methods of this invention is needed or desired. In some embodiments, the subject can be diagnosed with chronic fatigue syndrome and/or have symptoms of chronic fatigue syndrome or other fatigue-related disorder and in some embodiments the subject can have no diagnosis or symptom(s) of chronic fatigue syndrome or any other fatigue-related disorder. A subject of this invention that has an “increased likelihood” or “increased risk” of having or developing chronic fatigue syndrome can be a subject having symptoms and/or signs of chronic fatigue syndrome or other fatigue associated disorder or such a subject can be a subject who is not having symptoms and/or signs of chronic fatigue syndrome or other fatigue-associated disorder. A subject of this invention can have an increased risk or increased likelihood of having or developing chronic fatigue syndrome due to environmental and/or genetic factors as would be known to one of skill in the art. By “increased likelihood” or “increased risk” of having or developing chronic fatigue syndrome it is meant that the increase is relative to a control (e.g., a subject whose biomarker index is at or below the threshold biomarker index).

In the methods of this invention employing measurements over time, the time intervals can be minutes, hours, days, weeks, months and/or years in any order and in any combination.

In the methods of this invention that recite a first time point and a second or subsequent time points or later time points, in some embodiments, the first time point can be prior to performance of an activity (e.g., physical, athletic, mental, training, normal activity of daily living, etc.) and the second or subsequent time points can be during and/or after performance of the activity. In some embodiments, the first time point can be during and/or after the performance of activity and the second or subsequent points can be at a later time following completion of the activity.

Physical activity and/or athletic activity as described herein can be but is not limited to ultra-endurance exercise, a military operation, military training, running, walking, bicycling, weight lifting, swimming, a standardized physical test course including, for example, those used by the military, a triathlon, a biathlon, shooting of a rifle, shooting of a handgun, the aiming of computerized target equipment, staying awake, hiking, hiking while carrying a large burden on the back, physical activities of daily living and any combination thereof.

As used herein, the term “ultra-endurance exercise” means a single continuous session of physical activity and/or athletic activity during which the subject performs said activity for a minimum of four hours with an average exertion equal to or greater than 70% of ventalitory threshold, as described in Harger-Domitrovich et al., 2007, Medicine & Science in Sports & Exercise. For example, ten hours of continuous repetition of a one-hour exercise regimen consisting of 9 min. of upper-body ergometry, 19 min. of cycling, and 20 min. of treadmill walking with 1-min transition between modes, followed by a 10-min. rest and feeding period.

Military training is defined as the process of preparing military individuals and units to perform their assigned functions and missions, particularly to prepare for combat and wartime functions. “Covering every aspect of military activity, training is the principal occupation of military forces when not actually engaged in combat.” (Brassey's Encyclopedia of Land Forces and Warfare, By Franklin D. Margiotta). Training may include for example physical tasks such as swimming, hiking when equipped with full military gear, running, moving stealthily, climbing, performing the aforementioned tasks under extreme environmental conditions such as high and low temperatures, high altitude or under conditions where flora and fauna pose significant hazard.

Military operations are defined for the purposes of this instant invention as the activities engaged in by soldiers, sailors and airmen during performance of duties during periods of war and peace. Military operations may include tasks related to engagement of enemy combatants. These tasks may include, but are not limited to the following examples, pursuing the enemy on foot, flying unmanned drone aircraft, operating electronic equipment, manning guns in flight on an airplane, performing law enforcement duties and providing intelligence.

Normal activities of daily living, as defined by the National Cancer Institute, include eating, dressing, getting into or out of a bed or chair, taking a bath or shower, and using the toilet. Instrumental activities of daily living are activities related to independent living and include preparing meals, managing money, shopping, doing housework, and using a telephone.

The terms “fatigue” and “fatigued state” as used herein mean weariness or exhaustion from labor, exertion, exercise, or stress, including loss of physical strength and bodily and mental capabilities.

The term “chronic fatigue” as used herein describes fatigue lasting about six or more consecutive months, which is not due or identified to be due to ongoing exertion or other medical conditions associated with fatigue. “Chronic fatigue syndrome” or “CFS” as used herein describes the condition or status of a subject (e.g., a patient) who meets at least one of the criteria set forth for example, in 1) the CDC definition (1994) and also called the Fukuda definition, 2) The Oxford criteria (1991), which includes CFS of unknown etiology and a subtype called post-infectious fatigue syndrome (PIFS), 3) The 2003 Canadian Clinical working definition, which states that “[a] patient with ME/CFS will meet the criteria for fatigue, post-exertional malaise and/or fatigue, sleep dysfunction, and pain; have two or more neurological/cognitive manifestations and one or more symptoms from two of the categories of autonomic, neuroendocrine, and immune manifestations; and the illness will persist for at least 6 months,” 4) CFS/ME guideline for the National Health Service in England and Wales, produced in 2007 by the National Institute for Health and Clinical Excellence (NICE), and 5) other criteria including gene expression markers, genetic profiles and/or biomarkers as are known in the art. See, e.g., “Chronic Fatigue Syndrome/Myalgic Encephalomyelitis, A Primer for Clinical Practitioners” by the International Association for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (IACFS/ME), 2012 Edition, the entire contents of which are incorporated by reference herein. See also the following websites: www.iacfsme.org and www.cdc.gov/cfs/general/index.html, the entire contents of each of which are incorporated by reference herein.

A” treatment program for chronic fatigue syndrome” describes a physician-directed program including one or more of the treatments for Chronic Fatigue Syndrome recommended by the Centers for Disease Control and Prevention, International Association for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis, and/or other recognized organizations with expertise in Chronic Fatigue Syndrome

As used herein, “increasing the intensity of a treatment program” means changing a subject's treatment program such that the supervising physician believes that the level or intensity of future treatment is greater than the previous level or intensity of treatment.

Nonlimiting examples of treatments for chronic fatigue syndrome (CFS) include 1) cognitive behavioral therapy, 2) graded exercise therapy as a form of physical therapy, 3) pacing or energy management therapy, 4) pharmacotherapy including, e.g., Amitriptyline, Doxepin, Nortriptyline, Cyclobenzaprine, Trazodone, Gabapentin, Pregabalin, Promethazine, Diphenhydramine, Clonazepam, Orphenadrine, Ropinirole, pramipexole, Melatonin, Zolpidem, Zopiclone, Mirtazapine, Acetaminophen, Aspirin, Diclofenac, Gabapentin, Duloxetine, Codeine phosphate, oxycodone, hydrocodone, morphine, Tramadol, Modafinil, Armnodafanil, Methylphenindate, Dexamphetamine, Caffeine, Amphetamine salts Isoprinosine, anti-viral medications, rintatolimod, rituximab, antibiotics, anti-parasitics, dietary supplementation, vitamin D, vitamin B-12, B-complex vitamins, essential fatty acids, zinc and herbal remedies, light and radiation therapy, and any combination of these agents and therapies.

Also as used herein, the term “rested” or “rested state” or “non-fatigued state” means having sufficient rest from bodily and/or mental exertion, either before physical exercise and/or after recovery from fatigue.

“Perceived level of fatigue” as used herein means an individual's personal estimate or assessment of their fatigue and their ability to carry out tasks requiring a certain level of physical and/or cognitive performance

In addition, as used herein, “physical performance capability” means the capacity to accomplish a task which requires expenditure of a particular amount of energy. These tasks include, but are not limited to lifting objects, carrying objects, maintaining a certain pace of walking, running and/or cycling and maintaining a particular heart rate for a specified period. Capability represents a subject's potential for energy expenditure over a particular period of time.

The term “fitness” as used herein means good health or physical condition, especially as the result of exercise and proper nutrition.

In some embodiments of this invention, a subject of this invention is any animal in which identification of chronic fatigue syndrome, identification of increased likelihood or risk of having or developing chronic fatigue syndrome and/or identification of changes in fatigue level over time is needed or desired. In some embodiments, the subject is mammal and in particular embodiments the subject is a human. In other embodiments, the subject can be a horse, a dog or any other mammal about which the information obtained from the methods of this invention is needed or desired.

In some embodiments, the subject can be diagnosed with chronic fatigue syndrome and/or have symptoms of chronic fatigue syndrome or other fatigue-related disorder and in some embodiments the subject can have no diagnosis or symptom(s) of chronic fatigue syndrome or any other fatigue-related disorder. A subject of this invention that has an “increased likelihood” or “increased risk” of having or developing chronic fatigue syndrome can be a subject having symptoms and/or signs of chronic fatigue syndrome or other fatigue associated disorder or such a subject can be a subject who is not having symptoms and/or signs of chronic fatigue syndrome or other fatigue-associated disorder. A subject of this invention can have an increased risk or increased likelihood of having or developing chronic fatigue syndrome due to environmental and/or genetic factors as would be known to one of skill in the art. By “increased likelihood” or “increased risk” of having or developing chronic fatigue syndrome it is meant that the increase is relative to a control (e.g., a subject whose biomarker index is at or below the threshold biomarker index).

In some embodiments, a population of study subjects of this invention includes healthy male and/or female volunteers less than the age of 42 that are in good physical condition and not suffering from known diseases and/or healthy young (less than 25 years old) military members being screened for selection to Special Forces in the United States Military.

In some embodiments of this invention, a subject of this invention can be military personnel, a shift worker, a laborer, a truck driver, airline personnel, assembly line worker, a patient at a sleep clinic, an amateur athlete, a professional athlete, a worker in the oil and/or gas industry, a train driver, an astronaut, a space traveler, a coal miner, an air traffic controller and any combination thereof. A subject of this invention can also be any subject engaged in training and/or engaged in athletics, other employment or other activities outside of defined military duties. A subject of this invention may also be engaged in any work occupation as listed in the Standard Occupational Classification (SOC) System Manual, Version 2010, published by the US Department of Commerce and/or Brassey's Encyclopedia of Land Forces and Warfare, By Franklin D. Margiotta (1996) and/or The International Classification of Sleep Disorders Revised (2001) (ISBN-10: 157488087X) and/or “Sleep Disorders and Sleep Deprivation: An Unmet Public Health Problem” Harvey R. Colten and Bruce M. Altevogt, Editors, Committee on Sleep Medicine and Research, ISBN: 0-309-65727-X, 424 pages, (2006), the entire contents of each of which are incorporated by reference herein.

As used herein, a “sleep schedule” refers to periods of time allocated to sleep and taken at a specific time over some defined and recurring period. This can mean, as a non-limiting example, allocating a sleep period of 8 hours starting at 10 PM where the recurring period is 24 hours long where each recurring period starts at 6 AM. In another example one sleep period is 2 hours and starts at 4 PM and a second period starts at lAM and is 4 hours long where the recurring period is 24 hours long where each recurring period starts at 6 AM.

Guiding a subject's sleep schedule can be done by modifying the duration of the sleep periods, the number of sleep periods per day, and/or the time at which sleep periods are started. The recurring period may be determined, for example, by the subject's work schedule and/or any convenient recurring time pattern for example per day, week, month, etc.

Also as used herein, a “sleep enhancing material” refers to foods that are ingested, drugs that are administered by the oral route, intravenous route, transdermal route, intranasal route, via inhalation, by intra-ocular route, by vaginal route, and/or by rectal delivery route or other means, or complex mixtures that are delivered through a combination of routes in the form of a bath or immersion. Nonlimiting examples of a sleep enhancing material include an herbal tea, warm milk, turkey, a large meal, an alcoholic beverage, a dietary supplement, a mud bath, a salt bath, an FDA-approved drug agent with a specific indication for treatment of a sleep disorder including, e.g., insomnia, narcolepsy, sleep apnea, depression, anxiety, and sleep disorder associated with shift work.

Furthermore, a “sleep enhancing activity” refers to actions taken by the subject to promote sleepiness and thereby sleep, and/or actions taken by others directed towards the subject to promote sleepiness and thereby sleep, and/or actions taken by an automated system directed towards the subject to promote sleepiness and thereby sleep. Nonlimiting examples of a sleep enhancing activity include reading while lying down, reading poetry, watching television, listening to music, exposure to electromagnetic fields, having sex, soaking in warm water, massage, counting sheep and/or other redundant mental activity.

As used herein, “treatment of a sleep disorder” includes actions taken by a medical doctor or other medical practitioner on the subject to reduce symptoms associated with a sleep disorder or actions taken by a non-medical therapist or counselor on the subject to treat a sleep disorder or action taken by the spouse or friend or acquaintance of the subject to treat a sleep disorder or action taken by the subject to treat a sleep disorder. Nonlimiting examples of a treatment of a sleep disorder include taking an FDA approved agent and/or using a device for treatment of sleep disorder as prescribed by the subject's physician, psychological therapy delivered by a therapist to the subject to reduce anxiety and thereby mitigate sleep disorder, cognitive engagement by a spouse to improve marital relationship thereby reducing sleep disorder, a commitment and/or action on the part of the subject to work less thereby reducing sleep disorder. Enhancement of a treatment of a sleep disorder means increased intensity and/or frequency and/or dose and/or adding a new or additional treatment to an existing treatment regimen Reduction of a treatment of a sleep disorder means reduced intensity or frequency and/or dose and/or removing a treatment from an existing treatment regimen.

“Treatment of fatigue” as used herein means medical interventions and/or other physical and/or mental actions and/or changes in behavior to reduce fatigue. Nonlimiting examples of a treatment of fatigue include the use of medical interventions that may include drugs, surgery, and/or use of a approved medical device and/or approved medical therapy intended to reduce fatigue, the use of cognitive therapy and/or counseling to promote or elicit behaviors that reduce fatigue by reducing exposure to fatiguing activities and/or promoting activities that reduce fatigue. Specific non-limiting examples include taking a prescribed drug to promote restful sleep, thereby reducing fatigue, and/or engaging in fatigue mitigation counseling that leads, for example, to scheduling of more time for sleep.

As used herein, the term “work load” refers to cognitive and/or physical tasks that are required and/or desired for living. This includes tasks that are performed at home and outside the home. This includes, for example tasks related to activities of daily living and/or tasks accomplished or performed at a subject's workplace.

As also used herein, “fitness for duty” means having the ability to perform cognitive and/or physical tasks associated with daily living and/or work and having the ability to perform these tasks within reasonable periods of time and at a certain level of quality.

A “threshold ratio” describes a level of the ratio that is associated with a high likelihood of being able to perform a specific physical and/or cognitive task and/or to be fit for duty or to be in a non-fatigued state.

To perform a physical activity and/or athletic activity at a sufficient level means the ability to perform a task at a level that is required for professional advancement, required to pass a test, and/or required to complete a study. This also may include but is not limited to meeting individual personal physical and performance goals, satisfying job eligibility requirements, and/or meeting criteria required to continue working at a task, for example determining whether a person is too fatigued to drive a truck.

In some embodiments, to be “sufficiently rested to carry out a work load” includes having the ability to perform a task at a level that is required for professional advancement, required to pass a test and/or meet a predetermined threshold of performance and/or required to complete a study.

In some embodiments, a population of study subjects of this invention includes healthy male and/or female volunteers less than the age of 42 that are in good physical condition and not suffering from known diseases (e.g., determined not to have chronic fatigue syndrome) and/or healthy young (less than 25 years old) military members being screened for selection to Special Forces in the United States Military.

To perform a physical activity and/or athletic activity at a sufficient level means the ability to perform a task at a level that is required for professional advancement, required to pass a test, and/or required to complete a study. This also may include but is not limited to meeting individual personal physical and performance goals, satisfying job eligibility requirements, and/or meeting criteria required to continue working at a task, for example determining whether a person is too fatigued to drive a truck.

In some embodiments, to be “sufficiently rested to carry out a work load” includes having the ability to perform a task at a level that is required for professional advancement, required to pass a test and/or meet a predetermined threshold of performance and/or required to complete a study.

The term “chronic fatigue syndrome” as used herein describes an art-known syndrome, the signs and symptoms of which are described in the literature (see, e.g., Reeves et al. “Prevalence of chronic fatigue syndrome in metropolitan, urban, and rural Georgia” Population Health Metrics 5:5 (2007), the entire contents of which are incorporated by reference herein). Common symptoms and signs of chronic fatigue syndrome include fatigue, loss of memory or concentration, sore throat, enlarged lymph nodes in the neck and/or armpits, unexplained muscle pain, pain that moves from one joint to another without swelling or redness, headache of a new type, pattern or severity, unrefreshing sleep, and extreme exhaustion lasting more than 24 hours after physical or mental exercise. To meet the diagnostic criteria of chronic fatigue syndrome, a subject typically must have unexplained, persistent fatigue for six months or more, along with at least four of the following signs and symptoms: loss of memory or concentration, sore throat, enlarged lymph nodes in the neck or armpits, unexplained muscle pain, pain that moves from one joint to another without swelling or redness, headache of a new type, pattern or severity, unrefreshing sleep, and extreme exhaustion lasting more than 24 hours after physical or mental exercise.

The biomarkers and biomarker indices of this invention are correlated with chronic fatigue syndrome, fatigue, a fatigued state, an increase or decrease in fatigue, an increase or decrease in physical performance, a subject's perceived level of fatigue, recovery from a fatigued state, and/or an increased or decreased likelihood of performing an activity at a sufficient level as described herein according to methods well known in the art and as disclosed in the Examples provided herein. In general, identifying such correlation involves conducting analyses that establish a statistically significant association and/or a statistically significant correlation between the presence of a biomarker or biomarker index or a combination of biomarkers or biomarker indices and a change in the subject (e.g., from rested to fatigued state, during and/or after performance of physical activity or other defined or standardized activity) as detected according to standard methods. An analysis that identifies a statistical association (e.g., a significant association) between the biomarker or biomarker index or between the combination of biomarkers or biomarker indices and the change in the subject establishes a correlation between the increase or decrease of the biomarker or biomarker index or combination of biomarkers or biomarker indices in a subject and the change being analyzed.

It would be well understood by one of skill in the art that the methods of the present invention can be carried out on multiple subjects and the data compiled to produce mean and median values that indicate fatigue, a fatigued state, an increase or decrease in fatigue, an increase or decrease in physical performance, a subject's perceived level of fatigue, recovery from a fatigued state, and/or an increased or decreased likelihood of performing a physical activity at a sufficient level according to this invention. It would also be understood that the statistical limits described by the data obtained from groups of subjects can be applied to individual subjects' response. Thus, it would be understood that in some embodiments of this invention, the methods of this invention can be carried out using a computer, wherein, for example, the data from multiple subjects are stored in a computer database and analyzed according to art-known methods of statistical and mathematical analysis to identify means, medians, trends, statistically significant changes, variances, etc.

Thus, in some embodiments, the methods of this invention can be carried out using a computer. Thus the present invention provides a computer-assisted method of identifying fatigue, a fatigued state, an increase or decrease in fatigue, an increase or decrease in physical performance, a subject's perceived level of fatigue, recovery from a fatigued state, and/or an increased or decreased likelihood of performing a physical activity at a sufficient level. The method involves the steps of (a) storing a database of biological data for a plurality of subjects, the biological data that is being stored including for each of said plurality of subjects: (i) a description of the status of the subject and/or physical/athletic activity performed by the subject, (ii) a description of any performance enhancing material and/or activity administered to, contacted with and/or implemented by the subject; (iii) a description of measurements according to art-known methods detecting a change in the status or performance in the subject; and (iv) a description of measurements of biomarkers or biomarker indices in the subject; and then (b) querying the database to determine the relationship between a change in the measurement of biomarkers or biomarker indices in the subject and change in performance or status of the subject. Such querying can be carried out prospectively or retrospectively on the database by any suitable means, but is generally done by statistical analysis in accordance with known techniques, as described herein.

Compositions of the Invention

Further aspects of the present invention include an isolated peptide comprising, consisting essentially of, or consisting of the amino acid sequence of PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), an isolated peptide comprising, consisting essentially of, or consisting of the amino acid sequence of GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), and/or an isolated peptide comprising, consisting essentially of or consisting of the amino acid sequence of SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3) and a composition comprising any of these isolated peptides, singly or in any combination in a pharmaceutically acceptable carrier.

Also provided herein is an isolated peptide comprising, consisting essentially of or consisting of about five amino acids to about 15, 20, 25, 30, 35, 40, 45, 50, 50, 70, 80, 90 or 100 amino acids (including any value between 5 and 100 not explicitly recited herein), wherein the peptide comprises one or more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20) of the 5 mer peptides listed in Table 1, as well as a composition comprising any of these isolated peptides, singly or in any combination in a pharmaceutically acceptable carrier.

“Pharmaceutically acceptable,” as used herein, means a material that is not biologically or otherwise undesirable, i.e., the material may be administered to a subject along with the compositions of this invention, without causing substantial deleterious biological effects or interacting in a deleterious manner with any of the other components of the composition in which it is contained. The material would naturally be selected to minimize any degradation of the active ingredient and to minimize any adverse side effects in the subject, as would be well known to one of skill in the art (see, e.g., Remington's Pharmaceutical Science; latest edition). Exemplary pharmaceutically acceptable carriers for the compositions of this invention include, but are not limited to, sterile pyrogen-free water and sterile pyrogen-free physiological saline solution, as well as other carriers suitable for injection into and/or delivery to a subject of this invention, particularly a human subject, as would be well known in the art.

In some embodiments of this invention, a biomarker peptide and/or protein of this invention can be used, in any combination, as an antiviral, an antimicrobial, and/or an antifungal agent. Thus, the biomarker peptides and/or proteins of this invention can be used in methods of treating and/or preventing disorders such as disorders associated with fatigue (e.g., chronic fatigue syndrome), viral infection, disease associated with viral infection, microbial infection, disease associated with microbial infection, fungal infection, disease associated with fungal infection, and any combination thereof. Dosages, modes and regimens of administration for peptides and proteins as described herein would be determined by one of skill in the art according to art-known protocols (see, e.g., Remington's Pharmaceutical Science; latest edition).

In some embodiments, the present invention provides a biomarker protein or peptide of this invention, a nucleic acid comprising a nucleotide sequence encoding a biomarker protein or peptide of this invention, a vector comprising said nucleic acid and a cell containing said vector. The biomarker, the nucleic acid, the vector and/or the cell can be present singly and/or in any combination in a composition comprising a pharmaceutically acceptable carrier.

In other embodiments of this invention, a nucleic acid having the nucleotide sequence or a substantially similar nucleotide sequence of the gene encoding a biomarker protein or peptide of this invention can be used as a probe in a nucleic acid hybridization assay for the detection of nucleic acid encoding a biomarker protein or peptide in various tissues and/or body fluids of a subject of this invention. The probe can be used in any type of nucleic acid hybridization assay including Southern blots (Southern, 1975, J Mol. Biol. 98:508), Northern blots (Thomas et al., 1980, Proc. Natl Acad. Sci. U.S.A. 77:5201-05), colony blots (Grunstein et al., 1975, Proc. Natl Acad. Sci. U.S.A. 72:3961-65), slot blots, dot blots, etc. Stringency of hybridization can be varied depending on the requirements of the assay according to methods well known in the art. Assays for detecting nucleic acid encoding a protein in a cell, or the amount thereof, typically involve first contacting the cells or extracts of the cells containing nucleic acids therefrom with an oligonucleotide probe that specifically binds to nucleic acid encoding a protein or peptide as described herein (typically under conditions that permit access of the oligonucleotide to intracellular material), and then detecting the presence or absence of binding of the oligonucleotide probe thereto. Any suitable assay format can be employed (see, e.g., U.S. Pat. No. 4,358,535; U.S. Pat. Nos. 4,302,204; 4,994,373; 4,486,539; 4,563,419; and 4,868,104, the disclosures of each of which are incorporated herein by reference in their entireties).

As used herein, the terms peptide and polypeptide are used to describe a chain of amino acids, which correspond to those encoded by a nucleic acid. A peptide usually describes a chain of amino acids of from two to about 50 amino acids and polypeptide usually describes a chain of amino acids having more than about 50 amino acids. The term polypeptide can refer to a linear chain of amino acids or it can refer to a chain of amino acids, which have been processed and folded into a functional protein. It is understood, however, that 50 is an arbitrary number with regard to distinguishing peptides and polypeptides and the terms may be used interchangeably for a chain of amino acids around 50. The peptides and polypeptides of the present invention can be obtained by isolation and purification of the peptides and polypeptides from cells or body fluids or tissues where they are found naturally or by expression of a recombinant and/or synthetic nucleic acid encoding the peptide or polypeptide. The peptides and polypeptides of this invention can be obtained by chemical synthesis, by proteolytic cleavage of a polypeptide and/or by synthesis from nucleic acid encoding the peptide or polypeptide.

It is also understood that the peptides and polypeptides of this invention may also contain conservative substitutions where a naturally occurring amino acid is replaced by one having similar properties and which does not alter the function of the peptide or polypeptide. Such conservative substitutions are well known in the art. Thus, it is understood that, where desired, modifications and changes can be made in the nucleic acid sequence of the underlying gene(s) and/or amino acid sequence of the peptides and polypeptides of the present invention and still obtain a peptide or polypeptide having like or otherwise desirable characteristics. Such changes can occur in natural isolates or can be synthetically introduced using site-specific mutagenesis, the procedures for which, such as mismatch polymerase chain reaction (PCR), are well known in the art. One of skill in the art will also understand that polypeptides and nucleic acids that contain modified and/or synthetic amino acids and nucleotides, respectively (e.g., to increase the half-life and/or the therapeutic efficacy of the molecule), as are well known in the art, can be used in the methods of the invention.

“Nucleic acid” as used herein refers to single- or double-stranded molecules which may be DNA, comprised of the nucleotide bases A, T, C and G, or RNA, comprised of the bases A, U (substitutes for T), C, and G. The nucleic acid may represent a coding strand or its complement. Nucleic acids may be identical in sequence to a sequence that is naturally occurring or may include alternative codons that encode the same amino acid as that which is found in the naturally occurring sequence. Furthermore, nucleic acids may include codons that represent conservative substitutions of amino acids as are well known in the art. The nucleic acids of this invention can also comprise any nucleotide analogs and/or derivatives as are well known in the art.

As used herein, the term “isolated nucleic acid” means a nucleic acid separated or substantially free from at least some of the other components of the naturally occurring organism, for example, the cell structural components commonly found associated with nucleic acids in a cellular environment and/or other nucleic acids. The isolation of nucleic acids can therefore be accomplished by well-known techniques such as cell lysis followed by phenol plus chloroform extraction, followed by ethanol precipitation of the nucleic acids. The nucleic acids of this invention can be isolated from cells according to methods well known in the art for isolating nucleic acids. Alternatively, the nucleic acids of the present invention can be synthesized according to standard protocols well described in the literature for synthesizing nucleic acids. Modifications to the nucleic acids of the invention are also contemplated, provided that the essential structure and function of the peptide or polypeptide encoded by the nucleic acid are maintained.

The present invention further provides a kit for detection and/or quantification of the biomarkers of this invention. In some embodiments, such a kit can comprise one or more antibodies, ligands and/or aptamers, along with suitable buffers, wash solutions, dilution buffers, secondary antibodies, detection reagents, etc., for the detection of antigen/antibody complex formation, ligand/target complex formation and/or aptamer/target complex formation under various conditions. In another embodiment, a kit of this invention can comprise a nucleic acid probe or primer that is complementary to a nucleotide sequence encoding a biomarker of this invention, along with suitable buffers, wash solutions, dilution buffers, detection reagents, etc. for the amplification of target nucleic acid and/or detection of nucleic acid hybridization under various conditions.

Thus, in some embodiments, the present invention provides a kit comprising an antibody that specifically reacts with a biomarker of this invention and reagents for detecting antigen/antibody complex formation.

Further provided is a kit comprising an aptamer that specifically reacts with a biomarker of this invention and reagents for detecting aptamer/target molecule complex formation.

In addition, a kit is provided herein, comprising a nucleic acid that hybridizes under high stringency conditions with a nucleic acid encoding a biomarker of this invention and reagents for detecting nucleic acid hybridization complex formation.

Screening Methods

In addition, the present invention provides a method of identifying a substance that binds a peptide or protein of this invention, comprising contacting the peptide or protein with a test compound under conditions whereby binding between the peptide or protein and the test compound can be detected; and detecting binding between the peptide or protein and the test compound.

Further provided is a method of identifying a substance having the ability to inhibit or enhance the binding activity of a peptide or protein of this invention, comprising contacting the substance with the peptide or protein under conditions whereby binding can occur and detecting a decrease or increase in the amount of binding in the presence of the substance as compared to a control amount of binding in the absence of the substance, thereby identifying a substance having the ability to inhibit or enhance the binding activity of the peptide or protein.

For the methods of this invention that employ the detection of binding, such assays are well known in the art and can employ, for example, an antibody, ligand and/or aptamer that binds a peptide of this invention either directly or indirectly.

Also provided herein is a method of identifying a compound that modulates the activity of a peptide or protein of this invention, comprising contacting the peptide or protein with a test compound under conditions whereby modulation of the activity of the peptide or protein can be detected. Because there is an association between fatigue and a change in the levels of the peptides and proteins of this invention, the peptides and proteins may serve a role in, for example, communicating a state of high energy demand to target organs, altering function of organs involved in mobilization of energy, modulating the activity of organs involved with the mobilization of energy stores including adipose tissue, the liver and muscle, modulating the activity of gastrointestinal mucosal leading to increased absorption of sugars, converting amino acids to sugars, or modifying the metabolic and enzymatic activity of commensal bacteria residing in the gastrointestinal tract leading to increased availability of sugars and free fatty acids that can be used to accomplish physical work by voluntary muscle, modulating the activity of the liver, pancreas, duodenum and other organs that secrete enzymes, emulsifiers and other substances that affect the processing of food, altering the distribution and targeting of sugars, lipids and proteins in the blood. These activities can be measured using in vitro cell-based assays with various output functions that can be used to determine activity, cell-free assays that measure association with specific receptors or important regulatory molecules, gene expression assays, and methods that involve measurement of functional outputs or alterations of metabolic production, fat mobilization and other phenomenon associated with fatigue or the ability to perform physical and cognitive tasks.

Additionally, the present invention provides a method of identifying immunomodulating activity in a peptide or protein of this invention, specifically by employing the peptide or protein in an assay for immunomodulating activity and detecting immunomodulating activity in the presence of the peptide or protein as compared to a control, thereby identifying immunomodulating activity in the peptide or protein. In this method, the assay for immunomodulating activity can be, but is not limited to, antibody production (or other assay to detect humoral immune response, T cell activation (or other assay to detect cellular immune response), nitric oxide production, interleukin 2 (IL-2) secretion and any combination thereof.

Furthermore, a method is provided herein of identifying antiviral, antimicrobial and/or antifungal activity in a peptide or protein of this invention, comprising employing the peptide or protein in an assay for antiviral antimicrobial and/or antifungal activity and detecting antiviral, antimicrobial and/or antifungal activity in the presence of the peptide or protein as compared to a control, thereby identifying antiviral, antimicrobial and/or antifungal activity in the peptide or protein. Protocols for identifying antiviral, antimicrobial and/or antifungal activity in a substance are well known in the art.

The term “antibody” as used herein, includes, but is not limited to a polypeptide encoded by an immunoglobulin gene or immunoglobulin genes, or a fragment thereof “Antibody” also includes, but is not limited to, a polypeptide encoded by an immunoglobulin gene or immunoglobulin genes, or a fragment thereof, which specifically binds to and recognizes the biomarkers of this invention.

The term “epitope” means an antigenic determinant that is specifically bound by an antibody. Epitopes usually consist of surface groupings of molecules such as amino acids and/or sugar side chains and usually have specific three-dimensional structural characteristics, as well as specific charge characteristics.

The terms “specifically binds to” and “specifically reactive with” refer to a binding reaction that is determinative of the presence of the antigen and antibody or aptamer and target in the presence of a heterogeneous population of proteins, nucleic acids and/or other biologics. Thus, under designated assay conditions, the specified antibodies and antigens and/or aptamers and targets bind to one another and do not bind in a significant amount to other components present in a sample.

In some embodiments employing antibodies, a variety of immunoassay formats can be used to select antibodies specifically reactive with a particular antigen. For example, solid-phase ELISA immunoassays are routinely used to select monoclonal antibodies specifically immunoreactive with an analyte. See Harlow and Lane (ANTIBODIES: A LABORATORY MANUAL, Cold Springs Harbor Publications, New York, (1988)) for a description of immunoassay formats and conditions that can be used to determine specific immunoreactivity. Typically a specific or selective reaction will be at least twice background signal to noise and more typically more than 10 to 100 times greater than background.

An “immunologically reactive fragment” of a protein refers to a portion of the protein or peptide that is immunologically reactive with a binding partner, e.g., an antibody, which is immunologically reactive with the protein itself.

Antibodies to biomarkers of this invention can be generated using methods that are well known in the art. Such antibodies can include, but are not limited to, polyclonal, monoclonal, chimeric, humanized, fully human, single chain, Fab fragments, and/or fragments produced by an expression library, including e.g., phage display. (See, e.g., Paul, FUNDAMENTAL IMMUNOLOGY, 3rd Ed., 1993, Raven Press, New York, for antibody structure and terminology.)

Antibody fragments that contain specific binding sites for a biomarker of this invention can also be generated. For example, such fragments include, but are not limited to, the F(ab′)₂ fragments that can be produced by pepsin digestion of the antibody molecule, and the Fab fragments that can be generated by reducing the disulfide bridges of the F(ab′)₂ fragments. Alternatively, Fab expression libraries can be constructed to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity (Huse et al., Science 254, 1275-1281 (1989)).

For the production of antibodies, various hosts including goats, rabbits, rats, mice, humans, and others, may be immunized by injection with a protein or any fragment or oligopeptide or conjugate thereof that has immunogenic properties. Depending on the host species, various adjuvants can be used to increase the immunological response. Such adjuvants include, but are not limited to, Freund's complete and incomplete adjuvant, mineral gels such as aluminum hydroxide, and surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, and dinitrophenol. Examples of adjuvants used in humans include BCG (bacilli Calmette-Guerin) and Corynebacterium parvum.

Monoclonal antibodies can be prepared using any technique that provides for the production of antibody molecules by continuous cell lines in culture. These include, but are not limited to, the hybridoma technique, the human B-cell hybridoma technique, and the EBV-hybridoma technique (Kohler et al. (1975) Nature 256:495-497; Kozbor et al. (1985) J. Immunol. Methods 81:31-42; Cote et al. (1983) Proc. Natl. Acad. Sci. 80:2026-2030; Cole et al. (1984) Mol. Cell Biol. 62:109-120). Briefly, the procedure can be as follows: an animal is immunized with a protein or immunogenic fragment or oligopeptide or conjugate thereof. Lymphoid cells (e.g., splenic lymphocytes) are then obtained from the immunized animal and fused with immortalizing cells (e.g., myeloma or heteromyeloma) to produce hybrid cells. The hybrid cells are screened to identify those that produce the desired antibody.

Human hybridomas that secrete human antibody can be produced by the Kohler and Milstein technique and according to art-known protocols. Hybridoma production in rodents, especially mouse, is a very well established procedure and thus, stable murine hybridomas provide an unlimited source of antibody of select characteristics. As an alternative to human antibodies, the mouse antibodies can be converted to chimeric murine/human antibodies by genetic engineering techniques. See Oi et al. Bio Techniques 4(4):214-221 (1986); Sun et al. Hybridoma 5 (1986).

The monoclonal antibodies of this invention specific for biomarker epitopes of this invention can also be used to produce anti-idiotypic (paratope-specific) antibodies. (See e.g., McNamara et al., Science 220, 1325-26 (1984); Kennedy et al., Science 232:220 (1986).) These antibodies resemble the biomarker epitope and thus can be used as an antigen to stimulate an immune response against the biomarker.

In addition, techniques developed for the production of “chimeric antibodies,” the splicing of mouse antibody genes to human antibody genes to obtain a molecule with appropriate antigen specificity and biological activity can be used (Morrison et al. Proc. Natl. Acad. Sci. 81:6851-6855 (1984); Neuberger et al., Nature 312:604-608 (1984); Takeda et al., Nature 314:452-454 (1985)). Alternatively, techniques described for the production of single chain antibodies can be adapted, using methods known in the art, to produce biomarker protein-specific single chain antibodies. Antibodies with related specificity, but of distinct idiotypic composition, can be generated by chain shuffling from random combinatorial immunoglobin libraries (Burton, Proc. Natl. Acad. Sci. 88:11120-3 (1991)).

Antibodies can also be produced by inducing in vivo production in the lymphocyte population or by screening immunoglobulin libraries or panels of highly specific binding reagents as described in the literature (Orlandi et al., Proc. Natl. Acad. Sci. 86:3833-3837 (1989)); Winter et al., Nature 349:293-299 (1991)).

Various immunoassays can be used to identify biomarkers of this invention. Such immunoassays typically involve the measurement of antigen/antibody complex formation between a biomarker protein or peptide and its specific antibody.

The immunoassays of the invention can be either competitive or noncompetitive and both types of assays are well-known and well-developed in the art. In competitive binding assays, antigen or antibody competes with a detectably labeled antigen or antibody for specific binding to a capture site bound to a solid surface. The concentration of labeled antigen or antibody bound to the capture agent is inversely proportional to the amount of free antigen or antibody present in the sample.

Noncompetitive assays of this invention can be sandwich assays, in which, for example, the antigen is bound between two antibodies. One of the antibodies is used as a capture agent and is bound to a solid surface. The other antibody is labeled and is used to measure or detect the resultant antigen/antibody complex by e.g., visual or instrument means. A number of combinations of antibody and labeled antibody can be used, as are well known in the art. In some embodiments, the antigen/antibody complex can be detected by other proteins capable of specifically binding human immunoglobulin constant regions, such as protein A, protein L or protein G. These proteins are normal constituents of the cell walls of streptococcal bacteria. They exhibit a strong nonimmunogenic reactivity with immunoglobulin constant regions from a variety of species. (See, e.g., Kronval et al., J. Immunol., 111:1401-1406 (1973); Akerstrom et al., J. Immunol., 135:2589-2542 (1985).)

In some embodiments, the non-competitive assays need not be sandwich assays. For instance, the antibodies or antigens in the sample can be bound directly to the solid surface. The presence of antibodies or antigens in the sample can then be detected using labeled antigen or antibody, respectively.

In some embodiments, antibodies and/or proteins can be conjugated or otherwise linked or connected (e.g., covalently or noncovalently) to a solid support (e.g., bead, plate, slide, dish, membrane or well) in accordance with known techniques. Antibodies can also be conjugated or otherwise linked or connected to detectable groups such as radiolabels (e.g., ³⁵S, ¹²⁵I, ³²P, ¹³H, ¹⁴C, ¹³¹I), enzyme labels (e.g., horseradish peroxidase, alkaline phosphatase), gold beads, chemiluminescence labels, ligands (e.g., biotin) and/or fluorescence labels (e.g., fluorescein) in accordance with known techniques.

A variety of organic and inorganic polymers, both natural and synthetic can be used as the material for the solid surface. Nonlimiting examples of polymers include polyethylene, polypropylene, poly(4-methylbutene), polystyrene, polymethacrylate, poly(ethylene terephthalate), rayon, nylon, poly(vinyl butyrate), polyvinylidene difluoride (PVDF), silicones, polyformaldehyde, cellulose, cellulose acetate, nitrocellulose, and the like. Other materials that can be used include, but are not limited to, include paper, glass, ceramic, metal, metalloids, semiconductive materials, cements and the like. In addition, substances that form gels, such as proteins (e.g., gelatins), lipopolysaccharides, silicates, agarose and polyacrylamides can be used. Polymers that form several aqueous phases, such as dextrans, polyalkylene glycols or surfactants, such as phospholipids, long chain (12-24 carbon atoms) alkyl ammonium salts and the like are also suitable. Where the solid surface is porous, various pore sizes can be employed depending upon the nature of the system.

A variety of immunoassay systems can be used, including but not limited to, radio-immunoassays (RIA), enzyme-linked immunosorbent assays (ELISA) assays, enzyme immunoassays (EIA), “sandwich” assays, gel diffusion precipitation reactions, immunodiffusion assays, agglutination assays, immunofluorescence assays, fluorescence activated cell sorting (FACS) assays, immunohistochemical assays, protein A immunoassays, protein G immunoassays, protein L immunoassays, biotin/avidin assays, biotin/streptavidin assays, immunoelectrophoresis assays, precipitation/flocculation reactions, immunoblots (Western blot; dot/slot blot); immunodiffusion assays; liposome immunoassay, chemiluminescence assays, library screens, expression arrays, etc., immunoprecipitation, competitive binding assays and immunohistochemical staining. These and other assays are described, among other places, in Hampton et al. (Serological Methods, a Laboratory Manual, APS Press, St Paul, Minn. (1990)) and Maddox et al. (J. Exp. Med. 158:1211-1216 (1993); the entire contents of which are incorporated herein by reference for teachings directed to immunoassays).

The methods of this invention can also be carried out using a variety of solid phase systems, such as described in U.S. Pat. No. 5,879,881, as well as in a dry strip lateral flow system (e.g., a “dipstick” system), such as described, for example, in U.S. Patent Publication No. 20030073147, the entire contents of each of which are incorporated by reference herein.

In some embodiments, the biomarker of this invention can be detected and/or quantified in an assay employing an aptamer, a molecule that binds tightly to the biomarker in a manner similar to an antibody, a ligand or a small molecule. As used herein, the term “aptamer” includes any nucleic acid molecule or small peptide that specifically recognizes and binds a target molecule (e.g., a target peptide such as a biomarker of this invention). An “oligonucleotide-based aptamer” is defined as an aptamer made primarily, although not exclusively, from DNA and/or RNA bases. A “peptide-based aptamer” is defined as an aptamer made primarily, although not exclusively, from amino acids.

In some embodiments, an aptamer can be a small, usually stabilized, nucleic acid molecule that includes a binding domain for a target molecule (e.g., a biomarker of this invention). Oligonucleotide-based aptamers of this invention are oligonucleotides, or short (typically <100 bp) polymers of either DNA or RNA that have been selected from random pools based on their ability to bind nucleic acid, proteins, small organic compounds, and even entire organisms, usually with high affinity.

Oligonucleotide-based aptamers are typically developed to bind particular ligands using a previously described selection technique referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). This technique allows for selection of aptamers both in vivo and in vitro. Methods of making aptamers are described in several publications, for example, Ellington and Szostak, Nature 346:818 (1990), Tuerk and Gold, Science 249:505 (1990), U.S. Pat. No. 5,582,981, PCT Publication No. WO 00/20040, U.S. Pat. No. 5,270,163, Lorsch and Szostak, Biochemistry, 33:973 (1994), Mannironi et al., Biochemistry 36:9726 (1997), Blind, Proc. Nat'l. Acad. Sci. USA 96:3606-3610 (1999), Huizenga and Szostak, Biochemistry, 34:656-665 (1995), PCT Publication Nos. WO 99/54506, WO 99/27133, WO 97/42317 and U.S. Pat. No. 5,756,291.

Generally, in their most basic form, in vitro selection techniques for identifying oligonucleotide-based aptamers involve first preparing a large pool of oligonucleotides of the desired length that contain at least some central region that is randomized or mutagenized. For instance, a common oligonucleotide pool for aptamer selection might contain a region of 20-100 randomized nucleotides flanked on both ends by a relatively short (15-25 bp) region of nucleotides with defined sequence useful for the binding of PCR primers. The oligonucleotide pool is amplified using standard PCR techniques.

The original oligonucleotide pool is typically made of DNA bases. However, before the selection step, it can be converted to RNA bases using in vitro transcription methods well known in the art. During the selection step, the oligonucleotide library is allowed to interact with the target molecule, which is either free in solution or adhered to a physical surface such as a bead. In either case, the chemical environment of the interaction is typically controlled to simulate conditions anticipated for the final application of the invention, for example temperature, pH and osmolality matched to physiological conditions. When selection occurs in solution, capillary electrophoresis is used to separate bound from unbound oligonucleotides. For selection methods that use solid surfaces, bound and unbound oligonucleotide are separated by several rounds of washing of the surface. Bound oligonucleotide is isolated and amplified using standard PCR techniques. If the library was converted from DNA to RNA before selection, then reverse transcription must be used prior to PCR amplification. The amplified oligonucleotide sequences are then put through another round of the same type of selection. Typically, the selection process requires a total of three to ten iterative rounds to produce a high-affinity aptamer. In the final step, the amplified DNA is cloned and sequenced using standard procedures to identify the sequence of the oligonucleotides that are capable of acting as aptamers for the target molecule. Once a sequence has been identified for a tightly binding oligonucleotide-based aptamer, the nucleotide-based aptamer may be further refined and optimized for binding affinity by performing additional rounds of selection starting from a pool of oligonucleotides containing controlled levels of randomized mutations of the original oligonucleotide sequence.

In further embodiments, an oligonucleotide-based aptamer can include at least one modified nucleotide base. The term “modified nucleotide base” encompasses nucleotides with a covalently modified base and/or sugar. For example, modified nucleotides include nucleotides having sugars that are covalently attached to low molecular weight organic groups other than a hydroxyl group at the 3′ position and other than a phosphate group at the 5′ position. Such modified nucleotides can also include 2′ substituted sugars such as 2′-O-methyl; 2′-O-alkyl; 2′-O-allyl; 2′-S-alkyl; 2′-S-allyl; 2′-fluoro; 2′-halo; or 2′-azido-ribose, carbocyclic sugar analogues, a-anomeric sugars; epimeric sugars such as arabinose, xyloses or lyxoses, pyranose sugars, furanose sugars, and sedoheptulose.

Modified nucleotides of this invention can include but are not limited to, alkylated purines and/or pyrimidines; acylated purines and/or pyrimidines; and other heterocycles. These classes of pyrimidines and purines are known in the art and include, pseudoisocytosine; N4, N4-ethanocytosine; 8-hydroxy-N6-methyladenine; 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil; 5-fluorouracil; 5-bromouracil; 5-carboxymethylaminomethyl-2-thiouracil; 5-carboxymethylaminomethyl uracil; dihydrouracil; inosine; N6-isopentyl-adenine; 1-methyladenine; 1-methylpseudouracil; 1-methylguanine; 2,2-dimethylguanine; 2-methyladenine; 2-methylguanine; 3-methylcytosine; 5-methylcytosine; N6-methyladenine; 7-methylguanine; 5-methylaminomethyl uracil; 5-methoxy amino methyl-2-thiouracil; β-D-mannosylqueosine; 5-methoxycarbonylmethyluracil; 5-methoxyuracil; 2 methylthio-N6-isopentenyladenine; uracil-5-oxyacetic acid methyl ester; psuedouracil; 2-thiocytosine; 5-methyl-2 thiouracil, 2-thiouracil; 4-thiouracil; 5-methyluracil; N-uracil-5-oxyacetic acid methylester; uracil 5-oxyacetic acid; queosine; 2-thiocytosine; 5-propyluracil; 5-propylcytosine; 5-ethyluracil; 5-ethylcytosine; 5-butyluracil; 5-pentyluracil; 5-pentylcytosine; and 2,6-diaminopurine; methylpseudouracil; 1-methylguanine; and 1-methylcytosine.

Oligonucleotide-based aptamers of this invention can be synthesized from conventional phosphodiester linked nucleotides using standard solid or solution phase synthesis techniques that are known in the art. Linkages between nucleotides can use alternative linking molecules. For example, linking groups of the formula P(O)S, (thioate); P(S)S, (dithioate); P(O)NR′2; P(O)R′; P(O)OR6; CO; or CONR′2 wherein R is H (or a salt) or alkyl (1-12C) and R6 is alkyl (1-9C) is joined to adjacent nucleotides through —O— or —S—.

In certain embodiments, the present invention can employ monoclonal or polyclonal nucleotide-based aptamers. A “monoclonal nucleotide-based aptamer” as used herein includes a single aptamer with a known nucleotide sequence. A “polyclonal nucleotide-based aptamer” as used herein includes a population of aptamers with the same or different nucleotide sequences that all have an affinity for the same target molecule.

In other embodiments, an aptamer of this invention can be a recombinant protein or peptide that has been selected for specific binding to a target molecule according to methods known in the art (see, e.g., Hoppe-Seyler, Crnkovic-Mertens et al. 2004). The peptide-based aptamer can be a short peptide domain inserted into a supporting protein scaffold that enhances both specificity and affinity by conformationally constraining the peptide sequence (Colas, Cohen et al. 1996; Cohen, Colas et al. 1998; Buerger, Nagel-Wolfrum et al. 2003). In some embodiments of the present invention employing a peptide-based aptamer, the term “peptide-based aptamer” can be used to designate the peptide in the scaffold protein while the term “peptide” can refer to the inserted sequence.

In the methods of the present invention employing peptide-based aptamers, assays similar to the immunoassays described herein can be carried out to detect and/or quantify a biomarker of this invention, whereby a peptide-based aptamer is used in place of an antibody and an aptamer/target molecule complex, rather than an antibody/antigen complex is detected. The immunoassays described herein can also be adapted to employ an oligonucleotide-based aptamer in place of an antibody, for the detection of a nucleic acid/target molecule complex. In some embodiments, the immunoassays of this invention can also be modified to employ both aptamers and antibodies to detect and/or quantify a biomarker of this invention. Modification of any known immunoassay to accommodate the detection of binding of a nucleotide- or peptide-based aptamer to a target molecule would be well known to one of ordinary skill in the art.

As used herein, the term “signaling aptamer” includes aptamers with reporter molecules, such as a fluorescence dye, attached to the aptamer in such a way that upon conformational changes resulting from the interaction of the aptamer with a target molecule, the reporter molecule yields a differential signal, such as, for example, a change in fluorescence intensity. Alternatively, the amount of target molecule present may be quantified by the direct binding and retention of a fluorescently tagged aptamer on a solid surface or by the binding of a fluorescently tagged aptamer that recognizes the aptamer or antibody that binds specifically to the target molecule, i.e., secondary fluorescence assay. Examples of signaling aptamers can be found, for example, in U.S. Pat. No. 6,706,481, the entire contents of which are incorporated by reference herein for the disclosure of aptamers, methods of making aptamers and/or methods of using aptamers.

The present invention is more particularly described in the Examples set forth below, which are not intended to be limiting of the embodiments of this invention.

EXAMPLES Example 1 Discovery of Salivary Biomarkers for Chronic Fatigue Syndrome Sample Collection and Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis Sample Collection

Saliva samples were collected using a cotton swab-based collection system (Salivette, Sarstedt, Newton, N.C.). Samples were stored at −80° C., shipped on dry ice and processed according to the manufacturer's instructions.

Protein Content

The level of protein in each saliva sample was quantified using the colorimetric bicinchoninic assay (BCA). Absorbance measurements (562 nm) and standard solutions were used to construct a calibration curve and linear regression was used to determine the final protein concentration for the unknown sample.

Size-Based Centrifugal Filtration

The supernatant was spun through a 50 kDa molecular-weight cutoff (MWCO) filter (regenerated cellulose, Millipore, Billerica, Mass.) at 3,000 g for approximately 1 h at 22° C. The resulting filtrate was loaded into a 10 kDa MWCO filter (regenerated cellulose, Millipore) and spun for 1 h at 3,000 g at 22° C. The concentration of peptide and protein in the resulting filtrate was then determined using a commercially available kit for the BCA assay calibrated using bovine serum albumin standards. To remove salts and increase the concentration of peptides, ˜100 μg of protein was passed through a commercially available peptide trap (Michrom, Auburn, Calif.). The peptide concentration was again quantified using the BCA assay. The sample volume was then reduced using a heated centrifugal concentrator (Centrivap, Labconco, Kansas City, Mo.). Concentrated samples were labeled with a mass-specific variant of acetic anhydride, i.e., acetic anhydride with either methyl protons or methyl deuterons (Yu et al., 2004). The labeling mixture consisted of a 1:250 dilution of mass-specific acetic anhydride prepared in ethanol with 50 mM triethylammonium bicarbonate. Samples were incubated for 1 h at 37° C. and then concentrated as described above. Finally, two aliquots (˜2.5 μg each) from the same sample, labeled separately with light and heavy forms of acetic anhydride, were combined and injected onto the LC-MS system.

Ion-Trap Mass Spectrometry Detection

The various components of the processed saliva were separated using an ultra-high-pressure liquid chromatography (UPLC) system (Acquity, Waters, Milford, Mass.) with the outlet flowing directly into an ion-trap mass spectrometer (Bruker, Esquire 3000+, Billerica, Mass.). The UPLC was configured with a reversed-phase column (BEH300 C18, 1.7 μm particle, 2.1×100 mm, Waters) and the components were eluted from the column by varying the concentration of methanol in the running buffer linearly over a range from 10 to 35% at a flow rate of 0.3 ml/min. MS scans were collected at 2-5 Hz.

Analysis of LC-MS Data

A custom analysis program was written in LabVIEW (National Instruments, Austin, Tex.) to allow for the objective and automated identification of peak pairs within the data set separated by the expected mass-to-charge (m/z) differences appropriate for labeling with acetic anhydride, e.g., delta m/z of 3 for a singly charged ion that has been labeled with one acetate group, delta m/z of 6 for a singly charged ion that has been labeled with two acetate groups, etc. Because the overall number of ions identified in the CFS samples was lower than typical saliva samples examined previously, this analysis also included comparison of single ions, i.e., delta m/z of 0. Cluster analysis was used to identify peak pairs common to the group at the beginning and end of the study.

High-Resolution MS Sequencing of Salivary Peptides

To determine the amino acid sequence of ions of interest, fractions (˜1 min wide) of eluent near the elution time of the target ion were collected from LC injections of labeled saliva. Five fractions were pooled for high-resolution mass spectrometric analysis (12T LTQ-FT Ultra, ProSight PC).

Statistical Analysis

Statistical analysis was conducted using R (version 2.11.1) (Team 2010). For box plots, the horizontal line represents the median value while the boundaries of the rectangle indicate the range of the middle two quartiles. The whiskers indicate a distance 1.5× greater than the interquartile range from the nearest edge of the box. Open circles indicate points beyond the whiskers.

Results

Saliva samples were collected and analyzed as described above. The resulting LC-MS runs were analyzed with custom-written software, which enabled searches for clusters of mass-peak pairs within each group, i.e., searches for ions that were present in the LC-MS data for the majority of members of a particular group. Each LC-MS run was evaluated using the custom application and a list of detected mass pairs was written to a text file. In a typical LC-MS run, hundreds of thousands to millions of peaks were detected, of which several thousand were separated by one of the expected mass differences. The locations of these ion clusters were plotted with retention time serving as the x-coordinate and m/z serving as the y-coordinate.

From this plot of clusters, sites of potential biomarkers were identified, i.e., those sites for which a cluster appeared in one group, but not in the other. The coordinates of these sites were recorded and used to quantify the ion intensity at that location for each individual in the study. Data for three CFS-biomarker candidates are shown in FIGS. 1-3.

Quantifying Data Collected in an Ion Trap Mass Spectrometer

For the purposes of the study, the following approach for identifying biomarkers has been used. After the small-molecular-weight components of saliva were separated, the total protein concentration of the sample was estimated using a standard BCA assay. Using the estimated protein concentration, a total of 4 μg of protein was injected for each sample in an attempt to normalize the amount of material injected.

High-Resolution Mass Spectrometry Data

Amino acid sequence data for three peptides were obtained using high-resolution mass spectrometry. The high-resolution analysis returned the following sequences for the three peptides using the single-letter amino acid notation: (1) GNPQGPSPQGGNKPQGPPPPPGKPQ [bm_cfs_cand3], (2) PPGKPQGPPPQGGNQPQGPPPPPGKPQ [bm_cfs_cand4], and (3) SPPGKPQGPPQQEGNKPQGPPPPGKPQ [sp_(—)6]. The genes containing the sequences (1-3) are described below.

Genetic Information for Proteins Containing the Amino Acid Sequence of the Peptides

The Proline-rich Salivary Proteins (PRPs) constitute up to 70% of the soluble protein found in human saliva, and homologous proteins have been reported in non-human primates as well as in other animals, including rats, mice and hamsters. In humans, PRPs are the products of two gene families located on chromosome 12: (i) the HaeIII family, comprising two almost identical genes, PRH1 and PRH2, which code for acidic PRPs, and (ii) the BstN1 family, which includes four genes (PRB1, PRB2, PRB3 and PRB4) and codes for basic PRPs. With post-transcriptional and post-translational processing, these six genes are responsible for at least thirteen different human protein products. In addition, a number of allelic forms, representing minor changes in amino acid composition, have also been identified for each of these genes. A variety of functions have been suggested for PRPs in saliva including protection against bacterial pathogens, regulation of calcium phosphate deposition, and most recently as a protective mechanism against dietary tannins and other phenolic compounds.

The biomarker with the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ is derived from one of the PRB genes, PRB1, as a primary translation product containing 392 amino acids. These two PRB genes code for primary translation products of 392 amino acids (PRB1) and 416 amino acids (PRB2). Removal of the signal peptide produces Basic Salivary Proline-rich Protein 1 and Basic Salivary Proline-rich Protein 2, and further modifications yield several smaller products from each protein. Two of the final products of the PRB1 gene (Basic Salivary Proline-rich Protein 1 and Proline-rich Peptide II-2) contain amino acid sequence (1) [bm_cfs_cand3]. The method of release of the peptide into saliva is unclear. A detailed search of well-characterized proteases did not reveal any with enzymatic specificities that would generate this peptide fragment from the larger proteins.

The biomarker peptide with the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ derives from one, or perhaps both, of a pair of the basic proline-rich protein genes, PRB1 and PRB2, which are closely linked to the PRH genes. These two genes code for primary translation products of 392 amino acids (PRB1) and 416 amino acids (PRB2). Removal of the signal peptide produces Basic Salivary Proline-rich Protein 1 and Basic Salivary Proline-rich Protein 2, and further modifications yield several smaller products from each protein. Three of the final products of the PRB1 gene (Basic Salivary Proline-rich Protein 1, Proline-rich Peptide II-2 and Basic Peptide IB-6) and three from the PRB2 gene (Basic Salivary Proline-rich Protein 2, Basic Proline-rich Peptide IB-7 and Basic Proline-rich Peptide IB-8c) contain amino acid sequence (2) [bm_cfs_cand4]. As with sequence (1), it is as yet unclear whether only one or both of the PRB1 and PRB2 genes is the source of amino acid sequence (2).

The biomarker peptide with the amino acid sequence

SPPGKPQGPPQQEGNKPQGPPPPGKPQ derives from one of the basic proline-rich protein genes, PRB4. The gene codes for a primary translation products of 247 amino acids. Removal of the signal peptide produces Basic Salivary Proline-rich Protein 4, and further modifications yield several smaller products from each protein. Only one of the final products of the PRB4 gene (Basic Salivary Proline-rich Protein 4) contains amino acid sequence (3) [sp_(—)6].

Example 2 Measurement of Biomarker(s)

-   -   1. Collect a sample of saliva from the test subject.         -   a. Collect saliva by having the subject spit directly into a             collection vial or tube.         -   b. Collect saliva using a matrix-mediated approach, such as             the commercially available Salivette system developed by             Salimetrics.     -   2. Prepare sample for injection into liquid chromatography-mass         spectrometry (LC-MS) system.         -   a. For saliva collected as in (la):             -   i. Spin saliva at 4 k g for 45 min at 4° C.             -   ii. Determine protein concentration using bicinchoninic                 acid (BCA) assay with bovine serum albumin (BSA) as                 standard.             -   iii. Process saliva through molecular-weight-cutoff                 filters using 50 kDa and 10 kDa filters, sequentially.             -   iv. Pass final supernatant through peptide trap                 (Michrom, C8).             -   v. Determine protein concentration using BCA assay with                 BSA as standard.             -   vi. Dry the sample in a heated chamber with reduced                 pressure (Centrivap).             -   vii. Reconstitute the sample in water with 0.1% acetic                 acid at a concentration of 0.1 μg of protein/μl.             -   viii. Inject a sample containing 4 μg of protein, as                 determined by BCA assay, into LC-MS system.         -   b. For saliva collected as in (1b):             -   i. Extract raw saliva from matrix according to                 manufacturer's instructions.             -   ii. Follow steps (2. a.ii-vii)     -   3. Run LC-MS analysis using a linear gradient of acidified         (0.1%) water and methanol (95% to 65% water) to elute compounds         from a reversed-phase column.     -   4. Measure the height of the peak for peptides of interest, as         described herein.     -   5. Inject into the LC-MS system standard peptide solutions in at         least three different concentrations using concentration values         falling within the normal range of each peptide for 4 μg human         saliva.     -   6. Create a calibration curve for the peptide standards by         plotting peak height vs. concentration.     -   7. For each peptide of interest, use the standard curve results         in (6) and divide by 4 μg to determine the relative amount of         peptide per μg of total salivary protein, i.e., the biomarker         index for each peptide.

Example 3 Determining a Threshold Ratio

-   -   1. Using the measurement method described in Example 2, measure         the level of peptide in each sample collected from a group of         subjects (e.g., a population) determined not to have chronic         fatigue syndrome (non-CFS subjects). Such a determination can be         made for example as set forth in Reeves et al. (“Prevalence of         chronic fatigue syndrome in metropolitan, urban, and rural         Georgia” Population Health Metrics 5:5 (2007), the entire         contents of which are incorporated by reference herein).     -   2. Determine the median value of the peptide for the population         of non-CFS subjects.     -   3. Determine the standard deviation of the peptide for the         population of non-CFS subjects.     -   4. Set the threshold ratio for identifying a subject as having         chronic fatigue syndrome or having an increased likelihood of         having or developing chronic fatigue syndrome equal to the         following: Two times the standard deviation as determined in (3)         plus the median as determined in (2).

Example 4 Novel Salivary Biomarker Associated with Chronic Fatigue Syndrome

Background.

At present, diagnosis of chronic fatigue syndrome (CFS) requires a lengthy and expensive period of clinical examination to rule out all other causes of fatigue. Thus, many patients do not receive timely treatment or are misdiagnosed. Definitive rapid diagnosis is needed to improve the clinical outcomes for patients suffering from CFS.

Methodology/Principal Findings.

Saliva samples were obtained during a survey of the population of the state of Georgia (United States). The goal of this study was to determine incidence of undiagnosed CFS in the general population. Samples of saliva were maintained as a frozen archive until time of analysis. From the archive, samples from 46 subjects with CFS and 45 samples from normal controls were selected for further analysis. The ionizable components below 5 kDa were identified using liquid chromatography-mass spectrometry (LC-MS). Patterns of ion intensity as functions of retention time and mass-to-charge ratio were compared to identify significant differences between the CFS and control groups. A single biomarker candidate for CFS was identified. This biomarker candidate was approximately three times more abundant in saliva from CFS subjects than in saliva from control subjects (p<0.001, Wilcoxon's signed rank). The sensitivity and specificity of the biomarker candidate with respect to correctly identifying CFS are 88 and 91%, respectively. The Receiver Operating Characteristic (ROC) Area-Under-the-Curve (AUC) is 0.935 (95% CI 0.864 to 0.976). De novo sequencing by high-resolution MS revealed that the biomarker candidate was a peptide of molecular weight 2,633 Da. The amino acid sequence of the identified peptide is found within the sequence of the saliva-specific 42 kDa basic Proline-Rich-Protein, PRB4.

Conclusions/Significance.

A salivary peptide identified as a candidate biomarker for CFS may facilitate rapid diagnosis of CFS.

Chronic fatigue syndrome is an orphan disease wrapped in mystery. The etiology of the disease is not clear nor is its diagnosis and prognosis. Even the Fukuda definition, so frequently cited, describes CFS as unexplained fatigue [1]. The absence of clear etiological contributions is highlighted by the variety of labels for the condition including immune dysfunction syndrome, neuroendocrine immune dysfunction syndrome, allergic encephalomyelitis, post viral syndrome and neurasthenia, among others. The self-reported symptoms consistent with CFS include intense fatigue with a duration greater than six months, which is not relieved by rest and causes tiredness that impairs performance of daily activities. CFS is associated with a wide spectrum of symptoms including pain, headaches, cognitive disorders, sleep disorders, anxiety, depression and fatigue exacerbated by exercise. The search for effective treatments has been hampered by the lack of comprehension of the molecular and cellular basis for the development and progression of CFS.

The intense fatigue in CFS has been a cause of confusion with several other chronic conditions such as fibromyalgia, irritable bowel syndrome, and temporomandibular joint syndrome. A lengthy clinical evaluation, including a complete and detailed medical history, should be conducted to rule out other causes of fatigue and to characterize fatigue's form, time of onset, durations, triggering factors, relationship with rest and physical activities. The lack of objective criteria, specific signs and/or tests for the diagnosis of disease has led to an underestimation of CFS prevalence [2]. Although some have estimated that more than 800,000 people suffer from CFS in the US [3], causing a loss of $9 billion annually just in earnings and productivity [4], CDC has projected that only 9-16% of individuals with CFS had been diagnosed [5]. Even when detected, the average time from the beginning of the symptoms to the diagnosis of the syndrome is around 5 years [6]. Because the fatigue associated with CFS is so severe, patients with CFS are 4 times more likely to forgo needed healthcare than non-fatigued subjects [7], worsening the overall health outlook beyond the immediate impact of the disease itself.

Specific salivary peptides can be used to determine the physical fatigue status of athletes and adults during exercise [10]. Specifically the ratio of two endogenous peptides declined by approximately 1,000 fold from a rested state to a physically fatigued state over a period of several hours. Following rest, the peptide levels recovered. Both peptides are derived from a family of saliva specific proteins call Proline Rich Proteins (PRPs). These proteins and their associated peptides are only found in the saliva. In a separate study, the ratio of these peptides, termed the Fatigue Biomarker Index or FBI, was used to investigate physical fatigue levels of candidates for US military Special Forces [11]. These studies showed that a single measurement of the FBI made prior to the start of a rigorous selection process lasting 12 weeks was one of only four variables needed to predict who would ultimately pass and who would fail. In general, those candidates who failed, for reasons related to poor physical performance, had lower FBI levels, and thus higher levels of baseline fatigue than those individuals that passed. This suggests that levels of the FBI may indicate a physiological state of fatigue that is persistent and ultimately affects performance capability over a relatively long period of time. Taken together these findings suggested that saliva may provide an objective means of evaluating chronic fatigue.

In the current study, a comparative proteomic analysis directed at the low molecular weight, <5 kD, fraction of saliva was conducted. The goal of the study was to identify differences between CFS and control subjects. The results demonstrate that there is at least one peptide, derived from the family PRPs, which is significantly elevated in CFS patients relative to controls. This peptide offers promise as an objective diagnostic laboratory test for CFS.

Study Subjects

Study saliva samples were obtained from a large cross-sectional population based study of CFS and chronic un-wellness in Georgia, investigating the prevalence of CFS between September 2004 and July 2005 conducted by the Centers for Disease Control and Prevention[7]. Briefly, 10,837 households with 21,165 members were contacted initially by telephone interview. At the end of screening and selection a total of 112 participants met established clinical criteria for CFS using the criteria established by the 1994 international research case definition[1] using validated test instruments as specified by the International CFS Study Group [12] and CDC standards [13]. A total of 147 subjects identified as non-fatigued were identified during the Georgia study. The control subjects were clinically evaluated and saliva samples were obtained. Saliva samples were collected from these subjects and controls for the purpose of determining salivary cortisol levels [14]. In all cases, the sample obtained at 8 AM was used for the purpose of biomarker discovery. A total of 46 and 45 CFS and control saliva samples, respectively, were obtained for the purpose of biomarker discovery (FIG. 4). Samples were stored at −80° C. and shipped on dry ice until thawed for processing.

Processing of Saliva

Saliva samples were thawed at room temperature and spun for 10 minutes to remove particulates. The resulting supernatant was filtered sequentially through 50 kDa and 10 kDa molecular-weight cutoff filters (regenerated cellulose, Millipore, Billerica, Mass.) to produce a low molecular weight fraction of saliva. Approximately 100 ug of peptide, as determined by BCA, was desalted using a C-8 column designed for this purpose (Michrom, Auburn, Calif.).

Ion-Trap Mass Spectrometry to Identify Putative Biomarkers of CFS

Ion-trap mass spectrometers are capable of detecting components between a m/z of 150 to 2,000 with a precision of 0.1 m/z. The processed low-molecular weight saliva fraction was introduced onto an ultra-high-pressure liquid chromatography (UPLC) system (Acquity, Waters, Milford, Mass.) with the outlet flowing directly into an ion-trap mass spectrometer (Bruker, Esquire 3000+, Billerica, Mass.). The UPLC was configured with a reversed-phase column (BEH300 C18, 1.7 μm particle, 2.1×100 mm, Waters) and the components were eluted from the column by varying the concentration of methanol in the running buffer linearly over a range from 10 to 35% at a flow rate of 0.3 ml/min. MS scans were collected at 2-5 Hz. An analysis program (PeakQuest, Hyperion Biotechnology, San Antonio, Tex.) was used to identify putative biomarkers. PeakQuest enables identification of changes in the relative abundance of peptides. In this case, PeakQuest identifies component peptides that vary significantly in relative abundance compared to normal controls.

Definitive Chemical Identification of CFS Biomarkers

All putative biomarkers were found in a normal healthy pool of standard saliva (Hyperion Biotechnology Inc., San Antonio, Tex.). Portions of normal pool saliva were processed in the manner described and fractions 1 minute wide corresponding to the elution time were collected. Fractions were combined and concentrated and evaluated using high-resolution mass spectrometry (HRMS) (12T LTQ-FT Ultra, Pro Sight PC). HRMS provides a very precise estimation of peptides and peptide fragment mass, e.g., m/z±0.00001. During HRMS, peptide ions are fragmented by electron bombardment leading to specific disruption of peptide bonds. The disruption leads to the production of smaller peptides and individual amino acids obtained from the parent peptide. The mass of these resulting fragments is determined with great precision. The estimated masses acquired in this manner are then compared to theoretical masses that are found on extensive tables. The theoretical composition of the fragments enables a reconstruction of the parent peptide. This process is known as de novo sequencing. The de novo sequence determined in this manner can be confirmed by synthesizing the peptide called for from the de novo sequence. If the synthesized peptide demonstrates similar retention time and m/z on the system used for discovery (ion-trap instrument), the identity of the biomarker is confirmed. The de novo sequence data was used to direct synthesis of biomarker peptides. Milligram quantities of biomarkers were synthesized (Anaspec, Fremont, Calif.). The elution times and MS/MS fragmentation patterns of the synthesized peptides were observed and compared to similar data obtained from processed saliva.

Statistical Analysis

Statistical analysis was conducted using SYSTAT (SPSS Inc., Chicago, Ill.). The receiver operator characteristic (ROC) curve was calculated and analyzed using MedCalc ver. 12.2.1, (MedCalc Software, Mariakerke, Belgium). The standard error associated with ROC associated Area Under the Curve (AUC) was calculated using the method of DeLong [15]. Comparison between control and CFS was made by t-tests. Comparisons of distribution of subjects to control and CFS groups according to lifestyle, health factors and demographic factors were made using the Chi-square statistic.

A biomarker candidate for CFS was identified. Specifically, levels of the biomarker candidate were higher in CFS subjects than control as shown in FIG. 5. Setting a threshold value to 28,840 intensity units leads to a test with sensitivity of 91% and specificity of 88%. With regard to CFS, it is desirable to provide the highest possible confidence with regard to diagnosis. To increase confidence in positive diagnosis, a measured value above 35,000 provides 100% confidence of a diagnosis of CFS. However, at this level, 8 of 46 patients that are identified as having CFS based on clinical signs are incorrectly determined to be normal.

Subsequently, de novo sequencing of the putative biomarker using a high-resolution mass spectrometer led to chemical identification of the biomarker candidate, a peptide with the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ. The observed mass of the whole peptide was 2725.3907, which is in good agreement with the theoretical mass of 2725.3900 (p=6.88 E-73). A peptide of the same sequence was synthesized and tested on the original LC-MS system used for discovery studies. The retention time, m/z and fragmentation pattern of the synthesized peptide on the LC-MS system used for discovery were virtually identical to those observed for the ion of interest originally identified in saliva.

The amino acid sequence of the candidate CFS biomarker is found within the amino acid sequence of the human salivary protein, Basic Proline Rich Protein 4 (PRB4_HUMAN, P10163). This sequence is not found within any other known animal, bacterial or plant proteins. In humans, PBR4 is known to be expressed only in the parotid and other saliva glands.

Analysis was performed to determine if levels of the biomarker candidate were dependent on various demographic factors. Table 3 shows the distribution of subjects according to use of medication, gender, race, smoking, regularity of menstruation, menopause status, and obesity. Table 3 indicates that the CFS subjects are more likely to be taking medication, be smokers and have irregular menstruation than the normal subjects group. Table 3 shows that age and BMI are not statistically different between CFS and normal subjects.

Because the distribution of demographic factors is quite different between CFS and normal subjects, it may be argued that the CFS biomarker candidate identified in this study is instead a measure of factors related specifically to sampling differences. In other words, the biomarker candidate may be associated with medication usage, smoking or other demographic factors. To examine this possibility, CFS subjects were compared statistically using t-test. These results are shown in Table 4. Medication status, gender, race, smoking status, quality of menstruation, menopause status, and obesity were not associated with CFS biomarker level. Similarly, relationship of age and BMI to biomarker level was also examined. In this case no association was observed between these factors and level of the biomarker in CFS subjects. Taken together these observations suggest that the CFS biomarker is associated only with CFS and not with other factors examined here.

The diagnostic utility was evaluated through construction of a Receiver Operating Characteristic (ROC) curve. The ROC curve is shown in FIG. 6. The calculated Area-Under-the-Curve (AUC) of this ROC curve is 0.935 with a standard error of 0.0319 and with a 95% confidence interval using the binomial exact method of 0.864 to 0.976. A diagnostic test that has ROC AUC of 1.0 indicates a test that is able to perfectly discriminate between subjects with the disease and those that do not have the disease. A ROC AUC of 0.5 indicates a test that does no better than random assignment of individuals to groups.

The present study confirms that saliva can be used for detection and evaluation of disease-associated biomarkers and that CFS modulates the expression of a very specific set of molecules. A battery of biochemical and bioinformatic tools was employed to identify a 2.7 kDa peptide present in the salivary samples from CFS patients, at levels much higher than that of normal subjects. By identifying the amino acid sequence of this peptide and performing a search against the non-redundant GenBank® database, it was determined that the 2.7 kDa peptide is a fragment of human salivary proline-rich protein 4 (PRB4).

PRB4 belongs to the family of human salivary proline-rich proteins (PRPs), which include six closely linked genes on chromosome 12p13.2. All of the PRP genes are similar in structure, with complex electrophoretic patterns. Each PRP gene is approximately 4.0 kb in length and contains four exons, the third of which is entirely composed of 63-bp tandem repeats and encodes the proline-rich portion of the protein products. Exon 3 contains different numbers of tandem repeats in the different PRP genes. Variation in the numbers of these repeats is also responsible for length variations in different alleles of the PRB genes.

Currently, there are no specific biological or morphological biomarkers to establish per se the diagnosis of CFS. Its diagnosis is indefinite, and established through the exclusion of other diseases causing fatigue. Several studies have been conducted toward discovery of CFS biomarker(s), but the outcomes have been uncertain.

In conclusion, in the present study, a specific salivary biomarker was detected in subjects with CFS. Further studies are being designed to evaluate if the identified salivary 2.7 kDa peptide is not only a diagnostic biomarker but also a prognostic tool as well. In addition, other roles for this peptide, e.g., as a potential mediator of disease development and its progression, are currently being assessed.

The above examples clearly illustrate the advantages of the invention. Although the present invention has been described with reference to specific details of certain embodiments thereof, it is not intended that such details should be regarded as limitations upon the scope of the invention except as and to the extent that they are included in the accompanying claims.

Throughout this application, various patents, patent publications and non-patent publications are referenced. The disclosures of these patents, patent publications and non-patent publications in their entireties are incorporated by reference herein into this application in order to more fully describe the state of the art to which this invention pertains.

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Basic Proline-rich Protein 2 (PRB2; UniProt: P02812) MLLILLSVALLALSSAQNLNEDVSQEESPSLIAGNPQGAPPQGGNKPQGP PSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQG PPPQGDKSRSPRSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNKP QGPPPPGKPQGPPPQGDNKSRSSRSPPGKPQGPPPQGGNQPQGPPPPPGK PQGPPPQGGNKPQGPPPPGKPQGPPPQGDNKSQSARSPPGKPQGPPPQGG NQPQGPPPPPGKPQGPPPQGGNKSQGPPPPGKPQGPPPQGGSKSRSSRSP PGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQGPPP QGGSKSRSARSPPGKPQGPPQQEGNNPQGPPPPAGGNPQQPQAPPAGQPQ GPPRPPQGGRPSRPPQ 5-mer peptides of P02812 MLLIL SPPGK PQGDK PPPGK PPQGG PPPPP LLILL PPGKP QGDKS PPGKP PQGGN PPPPG LILLS PGKPQ GDKSR PGKPQ QGGNK PPPGK ILLSV GKPQG DKSRS GKPQG GGNKP PPGKP LLSVA KPQGP KSRSP KPQGP GNKPQ PGKPQ LSVAL PQGPP SRSPR PQGPP NKPQG GKPQG SVALL QGPPP RSPRS QGPPP KPQGP KPQGP VALLA GPPPQ SPRSP GPPPQ PQGPP PQGPP ALLAL PPPQG PRSPP PPPQG QGPPP QGPPP LLALS PPQGG RSPPG PPQGD GPPPP GPPPQ LALSS PQGGN SPPGK PQGDN PPPPG PPPQG ALSSA QGGNQ PPGKP QGDNK PPPGK PPQGG LSSAQ GGNQP PGKPQ GDNKS PPGKP PQGGN SSAQN GNQPQ GKPQG DNKSR PGKPQ QGGNK SAQNL NQPQG KPQGP NKSRS GKPQG GGNKS AQNLN QPQGP PQGPP KSRSS KPQGP GNKSQ QNLNE PQGPP QGPPP SRSSR PQGPP NKSQG NLNED QGPPP GPPPQ RSSRS QGPPP KSQGP LNEDV GPPPP PPPQG SSRSP GPPPQ SQGPP NEDVS PPPPP PPQGG SRSPP PPPQG QGPPP EDVSQ PPPPG PQGGN RSPPG PPQGD GPPPP DVSQE PPPGK QGGNQ SPPGK PQGDN PPPPG VSQEE PPGKP GGNQP PPGKP QGDNK PPPGK SQEES PGKPQ GNQPQ PGKPQ GDNKS PPGKP QEESP GKPQG NQPQG GKPQG DNKSQ PGKPQ EESPS KPQGP QPQGP KPQGP NKSQS GKPQG ESPSL PQGPP PQGPP PQGPP KSQSA KPQGP SPSLI QGPPP QGPPP QGPPP SQSAR PQGPP PSLIA GPPPQ GPPPP GPPPQ QSARS QGPPP SLIAG PPPQG PPPPP PPPQG SARSP GPPPQ LIAGN PPQGG PPPPG PPQGG ARSPP PPPQG IAGNP PQGGN PPPGK PQGGN RSPPG PPQGG AGNPQ QGGNK PPGKP QGGNQ SPPGK PQGGS GNPQG GGNKP PGKPQ GGNQP PPGKP QGGSK NPQGA GNKPQ GKPQG GNQPQ PGKPQ GGSKS PQGAP NKPQG KPQGP NQPQG GKPQG GSKSR QGAPP KPQGP PQGPP QPQGP KPQGP SKSRS GAPPQ PQGPP QGPPP PQGPP PQGPP KSRSS APPQG QGPPP GPPPQ QGPPP QGPPP SRSSR PPQGG GPPPP PPPQG GPPPP GPPPQ RSSRS PQGGN PPPPG PPQGG PPPPP PPPQG SSRSP QGGNK PPPGK PQGGN PPPPG PPQGG SRSPP GGNKP PPGKP QGGNK PPPGK PQGGN RSPPG GNKPQ PGKPQ GGNKP PPGKP QGGNQ SPPGK NKPQG GKPQG GNKPQ PGKPQ GGNQP PPGKP KPQGP KPQGP NKPQG GKPQG GNQPQ PGKPQ PQGPP PQGPP KPQGP KPQGP NQPQG GKPQG QGPPS QGPPP PQGPP PQGPP QPQGP KPQGP GPPSP GPPPQ QGPPP QGPPP PQGPP PQGPP PPSPP PPPQG GPPPP GPPPQ QGPPP QGPPP PSPPG PPQGD PPPPG PPPQG GPPPP GPPPQ PPPQG SARSP GRPSR PPQGG ARSPP RPSRP PQGGN RSPPG PSRPP QGGNQ SPPGK SRPPQ GGNQP PPGKP GNQPQ PGKPQ NQPQG GKPQG QPQGP KPQGP PQGPP PQGPP QGPPP QGPPQ GPPPP GPPQQ PPPPP PPQQE PPPPG PQQEG PPPGK QQEGN PPGKP QEGNN PGKPQ EGNNP GKPQG GNNPQ KPQGP NNPQG PQGPP NPQGP QGPPP PQGPP GPPPQ QGPPP PPPQG GPPPP PPQGG PPPPA PQGGN PPPAG QGGNK PPAGG GGNKP PAGGN GNKPQ AGGNP NKPQG GGNPQ KPQGP GNPQQ PQGPP NPQQP QGPPP PQQPQ GPPPP QQPQA PPPPG QPQAP PPPGK PQAPP PPGKP QAPPA PGKPQ APPAG GKPQG PPAGQ KPQGP PAGQP PQGPP AGQPQ QGPPP GQPQG GPPPQ QPQGP PPPQG PQGPP PPQGG QGPPR PQGGS GPPRP QGGSK PPRPP GGSKS PRPPQ GSKSR RPPQG SKSRS PPQGG KSRSA PQGGR SRSAR QGGRP RSARS GGRPS Basic Proline-rich Protein 3 (PRB3; UniProt: Q04118) MLLILLSVALLALSSAQSLNEDVSQEESPSVISGKPEGRRPQGGNQPQRT PPPPGKPEGRPPQGGNQSQGPPPRPGKPEGPPPQGGNQSQGPPPRPGKPE GQPPQGGNQSQGPPPRPGKPEGPPPQGGNQSQGPPPRPGKPEGPPPQGGN QSQGPPPHPGKPEGPPPQGGNQSQGPPPRPGKPEGPPPQGGNQSQGPPPR PGKPEGPPPQGGNQSQGPPPRPGKPEGSPSQGGNKPQGPPPHPGKPQGPP PQEGNKPQRPPPPGRPQGPPPPGGNPQQPLPPPAGKPQGPPPPPQGGRPH RPPQGQPPQ 5-mer peptides of Q04118 MLLIL PPPGK PPQGG GPPPH EGPPP KPQRP LLILL PPGKP PQGGN PPPHP GPPPQ PQRPP LILLS PGKPE QGGNQ PPHPG PPPQG QRPPP ILLSV GKPEG GGNQS PHPGK PPQGG RPPPP LLSVA KPEGR GNQSQ HPGKP PQGGN PPPPG LSVAL PEGRP NQSQG PGKPE QGGNQ PPPGR SVALL EGRPP QSQGP GKPEG GGNQS PPGRP VALLA GRPPQ SQGPP KPEGP GNQSQ PGRPQ ALLAL RPPQG QGPPP PEGPP NQSQG GRPQG LLALS PPQGG GPPPR EGPPP QSQGP RPQGP LALSS PQGGN PPPRP GPPPQ SQGPP PQGPP ALSSA QGGNQ PPRPG PPPQG QGPPP QGPPP LSSAQ GGNQS PRPGK PPQGG GPPPR GPPPP SSAQS GNQSQ RPGKP PQGGN PPPRP PPPPG SAQSL NQSQG PGKPE QGGNQ PPRPG PPPGG AQSLN QSQGP GKPEG GGNQS PRPGK PPGGN QSLNE SQGPP KPEGP GNQSQ RPGKP PGGNP SLNED QGPPP PEGPP NQSQG PGKPE GGNPQ LNEDV GPPPR EGPPP QSQGP GKPEG GNPQQ NEDVS PPPRP GPPPQ SQGPP KPEGS NPQQP EDVSQ PPRPG PPPQG QGPPP PEGSP PQQPL DVSQE PRPGK PPQGG GPPPR EGSPS QQPLP VSQEE RPGKP PQGGN PPPRP GSPSQ QPLPP SQEES PGKPE QGGNQ PPRPG SPSQG PLPPP QEESP GKPEG GGNQS PRPGK PSQGG LPPPA EESPS KPEGP GNQSQ RPGKP SQGGN PPPAG ESPSV PEGPP NQSQG PGKPE QGGNK PPAGK SPSVI EGPPP QSQGP GKPEG GGNKP PAGKP PSVIS GPPPQ SQGPP KPEGP GNKPQ AGKPQ SVISG PPPQG QGPPP PEGPP NKPQG GKPQG VISGK PPQGG GPPPR EGPPP KPQGP KPQGP ISGKP PQGGN PPPRP GPPPQ PQGPP PQGPP SGKPE QGGNQ PPRPG PPPQG QGPPP QGPPP GKPEG GGNQS PRPGK PPQGG GPPPH GPPPP KPEGR GNQSQ RPGKP PQGGN PPPHP PPPPP PEGRR NQSQG PGKPE QGGNQ PPHPG PPPPQ EGRRP QSQGP GKPEG GGNQS PHPGK PPPQG GRRPQ SQGPP KPEGP GNQSQ HPGKP PPQGG RRPQG QGPPP PEGPP NQSQG PGKPQ PQGGR RPQGG GPPPR EGPPP QSQGP GKPQG QGGRP PQGGN PPPRP GPPPQ SQGPP KPQGP GGRPH QGGNQ PPRPG PPPQG QGPPP PQGPP GRPHR GGNQP PRPGK PPQGG GPPPR QGPPP RPHRP GNQPQ RPGKP PQGGN PPPRP GPPPQ PHRPP NQPQR PGKPE QGGNQ PPRPG PPPQE HRPPQ QPQRT GKPEG GGNQS PRPGK PPQEG RPPQG PQRTP KPEGQ GNQSQ RPGKP PQEGN PPQGQ QRTPP PEGQP NQSQG PGKPE QEGNK PQGQP RTPPP EGQPP QSQGP GKPEG EGNKP QGQPP TPPPP GQPPQ SQGPP KPEGP GNKPQ GQPPQ PPPPG QPPQG QGPPP PEGPP NKPQR Basic Proline-rich Protein 4 (PRB4; UniProt: E9PAL0) MLLILLSVALLALSSAESSSEDVSQEESLFLISGKPEGRRPQGGNQPQRP PPPPGKPQGPPPQGGNQSQGPPPPPGKPEGRPPQGGNQSQGPPPHPGKPE RPPPQGGNQSQGTPPPPGKPERPPPQGGNQSHRPPPPPGKPERPPPQGGN QSQGPPPHPGKPEGPPPQEGNKSRSARSPPGKPQGPPQQEGNKPQGPPPP GKPQGPPPAGGNPQQPQAPPAGKPQGPPPPPQGGRPPRPAQGQQPPQ 5-mer peptides of E9PAL0 MLLIL PPPGK PPQGG GPPPH GPPPA LLILL PPGKP PQGGN PPPHP PPPAG LILLS PGKPQ QGGNQ PPHPG PPAGG ILLSV GKPQG GGNQS PHPGK PAGGN LLSVA KPQGP GNQSQ HPGKP AGGNP LSVAL PQGPP NQSQG PGKPE GGNPQ SVALL QGPPP QSQGT GKPEG GNPQQ VALLA GPPPQ SQGTP KPEGP NPQQP ALLAL PPPQG QGTPP PEGPP PQQPQ LLALS PPQGG GTPPP EGPPP QQPQA LALSS PQGGN TPPPP GPPPQ QPQAP ALSSA QGGNQ PPPPG PPPQE PQAPP LSSAE GGNQS PPPGK PPQEG QAPPA SSAES GNQSQ PPGKP PQEGN APPAG SAESS NQSQG PGKPE QEGNK PPAGK AESSS QSQGP GKPER EGNKS PAGKP ESSSE SQGPP KPERP GNKSR AGKPQ SSSED QGPPP PERPP NKSRS GKPQG SSEDV GPPPP ERPPP KSRSA KPQGP SEDVS PPPPP RPPPQ SRSAR PQGPP EDVSQ PPPPG PPPQG RSARS QGPPP DVSQE PPPGK PPQGG SARSP GPPPP VSQEE PPGKP PQGGN ARSPP PPPPP SQEES PGKPE QGGNQ RSPPG PPPPQ QEESL GKPEG GGNQS SPPGK PPPQG EESLF KPEGR GNQSH PPGKP PPQGG ESLFL PEGRP NQSHR PGKPQ PQGGR SLFLI EGRPP QSHRP GKPQG QGGRP LFLIS GRPPQ SHRPP KPQGP GGRPP FLISG RPPQG HRPPP PQGPP GRPPR LISGK PPQGG RPPPP QGPPQ RPPRP ISGKP PQGGN PPPPP GPPQQ PPRPA SGKPE QGGNQ PPPPG PPQQE PRPAQ GKPEG GGNQS PPPGK PQQEG RPAQG KPEGR GNQSQ PPGKP QQEGN PAQGQ PEGRR NQSQG PGKPE QEGNK AQGQQ EGRRP QSQGP GKPER EGNKP QGQQP GRRPQ SQGPP KPERP GNKPQ GQQPP RRPQG QGPPP PERPP NKPQG QQPPQ RPQGG GPPPH ERPPP KPQGP PQGGN PPPHP RPPPQ PQGPP QGGNQ PPHPG PPPQG QGPPP GGNQP PHPGK PPQGG GPPPP GNQPQ HPGKP PQGGN PPPPG NQPQR PGKPE QGGNQ PPPGK QPQRP GKPER GGNQS PPGKP PQRPP KPERP GNQSQ PGKPQ QRPPP PERPP NQSQG GKPQG RPPPP ERPPP QSQGP KPQGP PPPPP RPPPQ SQGPP PQGPP PPPPG PPPQG QGPPP QGPPP Basic Proline-rich Protein 4 (PRB4; UniProt: P10163) MLLILLSVALLALSSAESSSEDVSQEESLFLISGKPEGRRPQGGNQPQRP PPPPGKPQGPPPQGGNQSQGPPPPPGKPEGRPPQGGNQSQGPPPHPGKPE RPPPQGGNQSQGPPPHPGKPESRPPQGGHQSQGPPPTPGKPEGPPPQGGN QSQGTPPPPGKPEGRPPQGGNQSQGPPPHPGKPERPPPQGGNQSHRPPPP PGKPERPPPQGGNQSQGPPPHPGKPEGPPPQEGNKSRSARSPPGKPQGPP QQEGNKPQGPPPPGKPQGPPPPGGNPQQPQAPPAGKPQGPPPPPQGGRPP RPAQGQQPPQ 5-mer peptides of P10163 MLLIL PPPGK PPQGG GTPPP ERPPP KPQGP LLILL PPGKP PQGGN TPPPP RPPPQ PQGPP LILLS PGKPQ QGGNQ PPPPG PPPQG QGPPP ILLSV GKPQG GGNQS PPPGK PPQGG GPPPP LLSVA KPQGP GNQSQ PPGKP PQGGN PPPPG LSVAL PQGPP NQSQG PGKPE QGGNQ PPPGK SVALL QGPPP QSQGP GKPEG GGNQS PPGKP VALLA GPPPQ SQGPP KPEGR GNQSQ PGKPQ ALLAL PPPQG QGPPP PEGRP NQSQG GKPQG LLALS PPQGG GPPPH EGRPP QSQGP KPQGP LALSS PQGGN PPPHP GRPPQ SQGPP PQGPP ALSSA QGGNQ PPHPG RPPQG QGPPP QGPPP LSSAE GGNQS PHPGK PPQGG GPPPH GPPPP SSAES GNQSQ HPGKP PQGGN PPPHP PPPPG SAESS NQSQG PGKPE QGGNQ PPHPG PPPGG AESSS QSQGP GKPES GGNQS PHPGK PPGGN ESSSE SQGPP KPESR GNQSQ HPGKP PGGNP SSSED QGPPP PESRP NQSQG PGKPE GGNPQ SSEDV GPPPP ESRPP QSQGP GKPEG GNPQQ SEDVS PPPPP SRPPQ SQGPP KPEGP NPQQP EDVSQ PPPPG RPPQG QGPPP PEGPP PQQPQ DVSQE PPPGK PPQGG GPPPH EGPPP QQPQA VSQEE PPGKP PQGGH PPPHP GPPPQ QPQAP SQEES PGKPE QGGHQ PPHPG PPPQE PQAPP QEESL GKPEG GGHQS PHPGK PPQEG QAPPA EESLF KPEGR GHQSQ HPGKP PQEGN APPAG ESLFL PEGRP HQSQG PGKPE QEGNK PPAGK SLFLI EGRPP QSQGP GKPER EGNKS PAGKP LFLIS GRPPQ SQGPP KPERP GNKSR AGKPQ FLISG RPPQG QGPPP PERPP NKSRS GKPQG LISGK PPQGG GPPPT ERPPP KSRSA KPQGP ISGKP PQGGN PPPTP RPPPQ SRSAR PQGPP SGKPE QGGNQ PPTPG PPPQG RSARS QGPPP GKPEG GGNQS PTPGK PPQGG SARSP GPPPP KPEGR GNQSQ TPGKP PQGGN ARSPP PPPPP PEGRR NQSQG PGKPE QGGNQ RSPPG PPPPQ EGRRP QSQGP GKPEG GGNQS SPPGK PPPQG GRRPQ SQGPP KPEGP GNQSH PPGKP PPQGG RRPQG QGPPP PEGPP NQSHR PGKPQ PQGGR RPQGG GPPPH EGPPP QSHRP GKPQG QGGRP PQGGN PPPHP GPPPQ SHRPP KPQGP GGRPP QGGNQ PPHPG PPPQG HRPPP PQGPP GRPPR GGNQP PHPGK PPQGG RPPPP QGPPQ RPPRP GNQPQ HPGKP PQGGN PPPPP GPPQQ PPRPA NQPQR PGKPE QGGNQ PPPPG PPQQE PRPAQ QPQRP GKPER GGNQS PPPGK PQQEG RPAQG PQRPP KPERP GNQSQ PPGKP QQEGN PAQGQ QRPPP PERPP NQSQG PGKPE QEGNK AQGQQ RPPPP ERPPP QSQGT GKPER EGNKP QGQQP PPPPP RPPPQ SQGTP KPERP GNKPQ GQQPP PPPPG PPPQG QGTPP PERPP NKPQG QQPPQ Salivary acidic proline-rich phosphoprotein 1/2 (PRH1/PRH2; UniProt: P02810) MLLILLSVALLAFSSAQDLDEDVSQEDVPLVISDGGDSEQFIDEERQGPP LGGQQSQPSAGDGNQDDGPQQGPPQQGGQQQQGPPPPQGKPQGPPQQGGH PPPPQGRPQGPPQQGGHPRPPRGRPQGPPQQGGHQQGPPPPPPGKPQGPP PQGGRPQGPPQGQSPQ 5-mer peptides of P02810 MLLIL GGQQS PPQGR GRPQG LLILL GQQSQ PQGRP RPQGP LILLS QQSQP QGRPQ PQGPP ILLSV QSQPS GRPQG QGPPQ LLSVA SQPSA RPQGP GPPQG LSVAL QPSAG PQGPP PPQGQ SVALL PSAGD QGPPQ PQGQS VALLA SAGDG GPPQQ QGQSP ALLAF AGDGN PPQQG GQSPQ LLAFS GDGNQ PQQGG LAFSS DGNQD QQGGH AFSSA GNQDD QGGHP FSSAQ NQDDG GGHPR SSAQD QDDGP GHPRP SAQDL DDGPQ HPRPP AQDLD DGPQQ PRPPR QDLDE GPQQG RPPRG DLDED PQQGP PPRGR LDEDV QQGPP PRGRP DEDVS QGPPQ RGRPQ EDVSQ GPPQQ GRPQG DVSQE PPQQG RPQGP VSQED PQQGG PQGPP SQEDV QQGGQ QGPPQ QEDVP QGGQQ GPPQQ EDVPL GGQQQ PPQQG DVPLV GQQSQ PQQGG VPLVI QQQQG QQGGH PLVIS QQQGP QGGHQ LVISD QQGPP GGHQQ VISDG QGPPP GHQQG ISDGG GPPPP HQQGP SDGGD PPPPQ QQGPP DGGDS PPPQG QGPPP GGDSE PPQGK GPPPP GDSEQ PQGKP PPPPP DSEQF QGKPQ PPPPP SEQFI GKPQG PPPPG EQFID KPQGP PPPGK QFIDE PQGPP PPGKP FIDEE QGPPQ PGKPQ IDEER GPPQQ GKPQG DEERQ PPQQG KPQGP EERQG PQQGG PQGPP ERQGP QQGGH QGPPP RQGPP QGGHP GPPPQ QGPPL GGHPP PPPQG GPPLG GHPPP PPQGG PPLGG HPPPP PQGGR PLGGQ PPPPQ QGGRP LGGQQ PPPQG GGRPQ

TABLE 2 Basic Proline-rich Protein 1 (PRB1; UniProt: P04280) MLLILLSVALLALSSAQNLNEDVSQEESPSLIAGNPQGPSPQGGNKPQGP PPPPGKPQGPPPQGGNKPQGPPPPGKPQGPPPQGDKSRSPRSPPGKPQGP PPQGGNQPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQGPPPQGDKSQS PRSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQ GPPPQGDKSQSPRSPPGKPQGPPPQGGNQPQGPPPPPGKPQGPPQQGGNR PQGPPPPGKPQGPPPQGDKSRSPQSPPGKPQGPPPQGGNQPQGPPPPPGK PQGPPPQGGNKPQGPPPPGKPQGPPAQGGSKSQSARAPPGKPQGPPQQEG NNPQGPPPPAGGNPQQPQAPPAGQPQGPPRPPQGGRPSRPPQ

TABLE 3 Distribution of CFS and normal subjects with regard to demographic and other factors. Expressed as percentage where CFS n = 46 and normal subject n = 45. Chi-square statistic. Body weight is defined as normal, BMI between 18.5 to 24.9, overweight 25.0 to 29.9, and obese greater than 30 (www.cdc.gov/obesity/adult/defining.html). A smoker is defined as anyone who answered yes to the question “Have you ever smoked cigarettes regularly?” CFS Normal p-value Medication 0.001 Not Medicated 43.5 97.8 Medicated 56.5 2.2 Gender 0.059 Female 78.3 69.2 Male 21.7 30.8 Race 0.385 American Indian 4.3 0.0 Black 15.2 15.6 Other 2.2 0.0 White 78.3 84.4 Smoking 0.005 Smoker 67.4 37.8 Non-Smoker 32.6 62.2 Menstruation 0.016 Normal 16.7 44.4 Irregular 83.3 55.6 Menopause 0.109 Yes 61.1 40.7 No 38.9 59.3 Obesity 0.127 Normal 17.4 33.3 Overweight 39.1 40.0 Obese 43.5 26.7 Age 50.4 48.0 0.218 BMI 28.6 27.1 0.100

TABLE 4 Comparison of CFS subjects CFS biomarker level within group CFS Subjects compared Mean SD p-value Medication Not Medicated (n = 20) 52160 19727 0.465 Medicated (n = 26) 47736 20737 Gender Female (n = 36) 50684 21557 0.43 Male (n = 10) 45969 14632 Race White (n = 36) 49889 21060 0.875 Other (n = 10) 48833 17743 Smoking Smoker (n = 31) 47053 18888 0.25 Non-Smoker (n = 15) 55047 22387 Menstruation (female only) Irregular (n = 30) 55830 20599 0.525 Normal (n = 6) 49655 21935 Menopause (female only) Yes (n = 22) 48307 23014 0.397 No (n = 14) 54420 19262 Obesity Normal (n = 8) 52905 20554 0.633 Overweight or Obese (n = 38) 48976 20339 

1. A method of guiding a human subject's sleep schedule, comprising: a) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject; b) calculating the ratio of the concentration of the peptide(s) measured in (a) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); c) having the subject initiate or resume a sleep schedule; d) measuring the concentration of a peptide selected from the group consisting of: 1) a peptide comprising the amino acid sequence PPGKPQGPPPQGGNQPQGPPPPPGKPQ (SEQ ID NO:1), 2) a peptide comprising the amino acid sequence GNPQGPSPQGGNKPQGPPPPPGKPQ (SEQ ID NO:2), 3) a peptide comprising the amino acid sequence SPPGKPQGPPQQEGNKPQGPPPPGKPQ (SEQ ID NO:3), and 4) any combination thereof, in a saliva sample taken from the subject at one or more time points after (c), wherein the peptides of (d) are the same as the peptides of (a); e) calculating the ratio of the concentration of the peptide(s) measured in (d) to the total amount of protein in the sample, according to the equation: ([PPGKPQGPPPQGGNQPQGPPPPPGKPQ]+[GNPQGPSPQGGNKPQGPPPPPGKPQ]+[SPPGKPQGPPQQEGNKPQGPPPPGKPQ])/total protein (μg); and f) guiding the subject's sleep schedule by modifying the duration of subsequent sleep periods using the subject's ratio(s) as calculated in (e), such that an increase in the ratio relative to the previous ratio leads to a subsequent increase in the duration of the subject's sleep period, and a decrease in the ratio or a constant ratio relative to the previous ratio leads to no change in the duration of the subject's sleep period or a subsequent decrease in the duration of the subject's sleep period. 2-18. (canceled) 